Protein Information

Name Lipoxygenase (protein family or complex)
Synonyms arachidonate lipoxygenase; arachidonate lipoxygenases; lipoxygenase; lipoxygenases

Compound Information

Name chloroform
CAS trichloromethane

Reference List

PubMed Abstract RScore(About this table)
18237027 Ahmad B, Mukarram Shah SM, Khan H, Hassan Shah SM: Enzyme inhibition activities of Teucrium royleanum. J Enzyme Inhib Med Chem. 2007 Dec;22(6):730-2.

The crude methanolic extract and chloroform, ethyl acetate and n-butanol fractions of Teucrium royleanum were examined as inhibitors of actylcholinesterase, butyrylcholinesterase, lipoxygenase and urease.
82(1,1,1,2) Details
18237025 Khan H, Khan MA, Hussan I: Enzyme inhibition activities of the extracts from rhizomes of Gloriosa superba Linn (Colchicaceae). J Enzyme Inhib Med Chem. 2007 Dec;22(6):722-5.

The highest enzyme inhibition potency was expressed by the chloroform fraction (90%) among the tested fractions on lipoxygenase.
38(0,1,2,3) Details
17518351 Ahmad B, Shah SM, Bashir S, Shah J: Enzyme inhibition activities of Andrachne cardifolia Muell. J Enzyme Inhib Med Chem. 2007 Apr;22(2):235-8.

The crude methanolic extract and various fractions of Andrachne cardifolia Muell, including chloroform, ethyl acetate and n-butanol fractions were subjected to in vitro enzyme inhibition activity against acetylcholinesterase, butyrylcholinesterase, lipoxygenase and urease enzymes.
32(0,1,1,2) Details
19023788 Wahab A, Ahmed E, Nawaz SA, Sharif A, Ul Haq R, Malik A, Choudhary MI, Raza M: A pharmacological and toxicological evaluation of Haloxylon recurvum. Nat Prod Res. 2008;22(15):1317-26.

We investigated the in vivo toxic potential of crude methanolic extract of the whole plant and its n-hexane, chloroform, butanol, ethylacetate and aqueous soluble fractions by determining their acute toxicity and acute neurotoxicity in mice using Lorke's method and inverted screen test.
In in vitro lipoxygenase inhibition assay, the ethylacetate fraction showed the most significant inhibitory activity.
1(0,0,0,1) Details
17896957 Ghazaryan AV, Khatchadourian AS, Karagyozyan MK, Kachatryan AR, Sekoyan ES, Bdoyan HK, Melkumyan HV, Karageuzyan KG: Peculiarities of the anticoagulant properties for the newly synthesized preparations of coumarin-related compounds. Cardiovasc Hematol Disord Drug Targets. 2007 Sep;7(3):170-3.

B) DESIGN AND METHODS: Investigations were carried out on 120 white rats (180-200g), which were injected intravenously by 0.5 ml and 1.0 ml of 1 % solution of GSH-17 and decapitated after 10 and 30 min after the injection by use of light chloroform narcosis.
D) CONCLUSION: One of the possible mechanisms for haemostatic effect of the studied preparations, particularly GSH-17, probably can be accepted their effect on metabolism of arachidonic acid by lipoxygenase and cycloxygenase mechanisms [9, 10].
1(0,0,0,1) Details
18432845 Stenson WF: Measurement of prostaglandins and other eicosanoids. Curr Protoc Immunol. 2001 May;Chapter 7:Unit 7.33.

Eicosanoids are arachidonic acid metabolites formed through the cyclooxygenase or one of the lipoxygenase metabolic pathways.
A method is also described that can be used to analyze the full range of eicosanoids produced in a biological system, and a support protocol presents an acetone/chloroform extraction method for purifying eicosanoids prior to assay.
1(0,0,0,1) Details