Protein Information

Name hemolysin
Synonyms CBP; CBP; Csk binding protein; PAG; PAG 1; PAG1; Phosphoprotein associated with glycosphingolipid enriched microdomains; Phosphoprotein associated with glycosphingolipid enriched microdomains 1…

Compound Information

Name sodium azide
CAS sodium azide

Reference List

PubMed Abstract RScore(About this table)
8023897 Trinkle-Mulcahy L, Siegman MJ, Butler TM: Metabolic characteristics of alpha-toxin-permeabilized smooth muscle. . Am J Physiol. 1994 Jun;266(6 Pt 1):C1673-83.

Treatment of the intact muscle with the ectonucleotidase inhibitor 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS) followed by alpha-toxin permeabilization and inclusion of sodium azide in subsequent solutions reduces the ecto-ATPase by approximately 70%.
35(0,1,1,5) Details
8828128 Amoako KK, Goto Y, Xu DL, Shinjo T: The effects of physical and chemical agents on the secretion and stability of a Fusobacterium necrophorum hemolysin. Vet Microbiol. 1996 Jul;51(1-2):115-24.

However, the addition of sodium azide, an energy metabolic inhibitor, significantly reduced the hemolytic activity.
4(0,0,0,4) Details
8662777 Skiba PJ, Zha X, Maxfield FR, Schissel SL, Tabas I: The distal pathway of lipoprotein-induced cholesterol esterification, but not sphingomyelinase-induced cholesterol esterification, is energy-dependent. J Biol Chem. 1996 Jun 7;271(23):13392-400.

The esterification pathway can also be stimulated by hydrolysis of cell-surface sphingomyelin by the enzyme sphingomyelinase (SMase).
After the atherogenic lipoprotein, beta-very low density lipoprotein (beta-VLDL), was internalized by macrophages and hydrolyzed in lysosomes, the cells were depleted of energy by treatment with sodium azide and 2-deoxyglucose or by permeabilization.
3(0,0,0,3) Details