Protein Information

Name alpha L fucosidase
Synonyms Alpha L fucosidase; Alpha L fucosidase I; Alpha L fucoside fucohydrolase; FUCA; FUCA 1; FUCA1; Fucosidase; Tissue alpha L fucosidase…

Compound Information

Name sodium azide
CAS sodium azide

Reference List

PubMed Abstract RScore(About this table)
12911294 Cobucci-Ponzano B, Trincone A, Giordano A, Rossi M, Moracci M: Identification of the catalytic nucleophile of the family 29 alpha-L-fucosidase from Sulfolobus solfataricus via chemical rescue of an inactive mutant. Biochemistry. 2003 Aug 19;42(32):9525-31.

We describe here the identification of the catalytic nucleophile of the reaction in the alpha-L-fucosidase from S. solfataricus by reactivation with sodium azide of the mutant Asp242Gly that shows a 10 (3)-fold activity reduction on pNp-Fuc.
34(0,1,1,4) Details
15835922 Cobucci-Ponzano B, Mazzone M, Rossi M, Moracci M: Probing the catalytically essential residues of the alpha-L-fucosidase from the hyperthermophilic archaeon Sulfolobus solfataricus. Biochemistry. 2005 Apr 26;44(16):6331-42.

Instead, the Ssalpha-fucGlu58Gly mutant revealed a 4000-fold reduction of k (cat)/K (M) if compared to the wild-type and showed the rescue of the k (cat) by sodium azide at wild-type levels.
3(0,0,0,3) Details