| Name | beta glucuronidase |
|---|---|
| Synonyms | Beta glucuronidase; Beta G1; Beta glucuronidase precursor; GUSB; MPS 7; MPS7; Beta G1s; Beta glucuronidase precursors |
| Name | 2,4-D |
|---|---|
| CAS | 2-(2,4-dichlorophenoxy)acetic acid |
| PubMed | Abstract | RScore(About this table) | |
|---|---|---|---|
| 16667958 | Fujii N, Uchimiya H: Conditions Favorable for the Somatic Embryogenesis in Carrot Cell Culture Enhance Expression of the roIC Promoter-GUS Fusion Gene. Plant Physiol. 1991 Jan;95(1):238-241. Upon transferring the cells from a 2,4-D-containing medium to one devoid of 2,4-dichlorophenoxyacetic acid, enhanced expression of beta-glucuronidase in somatic embryo development was recorded. |
34(0,1,1,4) | Details |
| 18500684 | Lee CY, Agrawal DC, Wang CS, Yu SM, Chen JJ, Tsay HS: T-DNA activation tagging as a tool to isolate Salvia miltiorrhiza transgenic lines for higher yields of tanshinones. Planta Med. 2008 Jun;74(7):780-6. Epub 2008 May 26. Of these 1435 ATM cell lines, six lines (T1-T6) showed a red color on a selective medium containing 4.5 microM 2,4-dichlorophenoxyacetic acid (2,4-D), which is used as a phenotypic model system to identify the accumulation of tanshinones. 700 out of 1435 ATM cell lines were tested with a beta-glucuronidase (GUS) assay, 35 showed GUS activity. |
6(0,0,1,1) | Details |
| 9202719 | Griffin RJ, Salemme J, Clark J, Myers P, Burka LT: Biliary elimination of oral 2,4-dichlorophenoxyacetic acid and its metabolites in male and female Sprague-Dawley rats, B6C3F1 mice, and Syrian hamsters. J Toxicol Environ Health. 1997 Jul;51(4):401-13. This was tentatively identified as 2,4-D- based on its hydrolysis by beta-glucuronidase. |
6(0,0,1,1) | Details |
| 11448754 | Jaiwal PK, Kumari R, Ignacimuthu S, Potrykus I, Sautter C: Agrobacterium tumefaciens-mediated genetic transformation of mungbean (Vigna radiata L. Plant Sci. 2001 Jul;161(2):239-247. Hypocotyl and primary leaves excised from 2-day-old in-vitro grown seedlings produced transgenic calli on B (5) basal medium supplemented with 5x10 (-6) M BAP, 2.5x10 (-6) M each of 2,4-D and NAA and 50 mg l (-1) kanamycin after co-cultivation with Agrobacterium tumefaciens strains, LBA4404 (pTOK233), EHA105 (pBin9GusInt) and C58C1 (pIG121Hm) all containing beta-glucuronidase (gusA) and neomycin phosphotransferase II (nptII) marker genes. |
6(0,0,1,1) | Details |
| 11332725 | Nikovics K, Simidjieva J, Peres A, Ayaydin F, Pasternak T, Davies JW, Boulton MI, Dudits D, Horvath GV: Cell-cycle, phase-specific activation of Maize streak virus promoters. Mol Plant Microbe Interact. 2001 May;14(5):609-17. Histochemical staining of plant roots containing "long and short" C-sense promoter sequences upstream of the GUS (beta-glucuronidase) reporter gene showed that promoter activity was restricted to the meristematic region of the roots and was enhanced by 2,4-dichlorophenoxy (2,4-D) treatment. |
6(0,0,1,1) | Details |
| 8016259 | Fujii N, Yokoyama R, Uchimiya H: Analysis of the rolC promoter region involved in somatic embryogenesis-related activation in carrot cell cultures. Plant Physiol. 1994 Apr;104(4):1151-7. In cell cultures of carrot (Daucus carota L.), somatic embryogenesis can be induced by transferring cells from a medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) to one devoid of 2,4-D. Previous analysis of transgenic carrot cells containing the 5' non-coding sequence of the Ri plasmid rolC and a structural gene for bacterial beta-glucuronidase (uidA) has shown that the chimeric gene is actively expressed after induction of somatic embryogenesis. |
2(0,0,0,2) | Details |
| 20039139 | Xu ZQ, Huang X, Feng C, Tian N, Xu D, Feng SZ: Multicellular genesis of leaf primordium was demonstrated via chimaeric transgenic plant of maize (Zea mays L.) regenerated from Type II calli. Mol Biol Rep. 2009 Dec 29. Type-II embryonic calli were induced from immature embryos of maize (Zea mays L.) genotype YD and bombarded with beta-glucuronidase gene. Bombarded calli were proliferated on normal N6 medium for 2 weeks at 26 degrees C in the dark and selected on N6 medium containing 1 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 5 mg/l phosphinothricin (PPT) but without casamino acids and under the same conditions for 14 days. |
2(0,0,0,2) | Details |
| 12654044 | Mathieu M, Neutelings G, Hawkins S, Grenier E, David H: Cloning of a pine germin-like protein (GLP) gene promoter and analysis of its activity in transgenic tobacco Bright Yellow 2 cells. Physiol Plant. 2003 Mar;117(3):425-434. In Pinus caribaea Morelet, the PcGER1 gene is expressed uniquely in embryo tissues, and encodes a GLP ionically bound to the walls of pine embryo cells maintained in 2,4-D-containing medium. The PcGER1 promoter sequence was cloned upstream of the GUS (beta-glucuronidase) reporter gene and transferred to tobacco Bright Yellow 2 (BY-2) cells via Agrobacterium tumefaciens-mediated transformation. |
1(0,0,0,1) | Details |
| 8979515 | Ramana RV, Venu C, Jayasree T, Sadanadam A: Direct somatic embryogenesis and transformation in Cicer arietinum L. Indian J Exp Biol. 1996 Jul;34(7):716-8. Somatic embryos were induced directly from immature cotyledons of the genotype of chickpea ICC 4918 (annigiri) on B5 medium supplemented with 2,4,5-T or 2,4-D in combination with BA or KN. Successful transformation was achieved via somatic embryogenesis using Agrobacterium tumefaciens strain LBA4404, carrying a binary plasmid vector system containing neomycin phosphotransferase (NPT II) gene as the selectable marker and beta-glucuronidase (GUS) as a reporter gene. |
1(0,0,0,1) | Details |
| 11760772 | Egnin M, Mora A, Prakash CS: Factors enhancing Agrobacterium tumefaciens-mediated gene transfer in peanut (Arachis hypogaea L.). In Vitro Cell Dev Biol Plant. 1998 Oct-Dec;34(4):310-8. An intron-containing beta-glucuronidase uidA (gusA) gene under the transcriptional control of CaMV 35S promoter served as a reporter. The use of cocultivation media containing high auxin [1.0 or 2.5 mg/l (4.53 micromolar or 11.31 micromolar) 2,4-D] and low cytokinin [0.25 or 0.5 mg/l (1.0 micromolar or 2.0 micromolar) BA] promoted higher transformation than either hormone-free or thidiazuron-containing medium. |
1(0,0,0,1) | Details |
| 8704145 | Cheng JC, Seeley KA, Goupil P, Sung ZR: Expression of DC8 is associated with, but not dependent on embryogenesis. Plant Mol Biol. 1996 Apr;31(1):127-41. To investigate the mechanism of DC8 expression during seed development, chimeric gene constructs containing DC8 promoter fragments fused to a promoterless beta-glucuronidase gene (DC8:GUS) were introduced into carrot, tobacco (Nicotiana tobacum) and Arabidopsis thaliana plants. A similar DC8 activity time-course during callus induction and seed development suggests that explantation and 2,4-D treatment initiates a course of events similar to that in the carrot ovule. |
1(0,0,0,1) | Details |
| 15889272 | Rock CD, Sun X: Crosstalk between ABA and auxin signaling pathways in roots of Arabidopsis thaliana (L.) Heynh. Planta. 2005 Sep;222(1):98-106. Epub 2005 May 12. The Daucus carota (L.) Dc3 promoter: uidA (beta-glucuronidase: GUS) chimaeric reporter (ProDc3:GUS) is induced by ABA, osmoticum, and the auxin (IAA) in vegetative tissues of transgenic Arabidopsis thaliana (L.) Heynh. |
1(0,0,0,1) | Details |
| 14551731 | Cho MJ, Yano H, Okamoto D, Kim HK, Jung HR, Newcomb K, Le VK, Yoo HS, Langham R, Buchanan BB, Lemaux PG: Stable transformation of rice (Oryza sativa L.) via microprojectile bombardment of highly regenerative, green tissues derived from mature seed. Plant Cell Rep. 2004 Feb;22(7):483-9. Epub 2003 Oct 10. These tissues were induced from mature seeds on NB-based medium containing 2,4-dichlorophenoxyacetic acid (2,4-D), 6-benzylaminopurine (BAP) and high concentrations of cupric under dim light conditions; germinating shoots and roots were completely removed. From 431 explants bombarded with transgenes [i.e. a hygromycin phosphotransferase ( hpt) gene plus one of a wheat thioredoxin h ( wtrxh), a barley -thioredoxin reductase ( bntr), a maize Mutator transposable element ( mudrB) or beta-glucuronidase ( uidA; gus) gene], 28 independent transgenic events were obtained after an 8- to 12-week selection period, giving a 6.5% transformation frequency. |
1(0,0,0,1) | Details |
| 18057938 | Facchini PJ, Loukanina N, Blanche V: Genetic transformation via somatic embryogenesis to establish herbicide-resistant opium poppy. Plant Cell Rep. 2008 Apr;27(4):719-27. Epub 2007 Dec 5. Transformation was mediated by Agrobacterium tumefaciens using the pCAMBIA3301 vector, which harbors the phosphinothricin acetyltransferase (pat) gene driven by a tandem repeat of the cauliflower mosaic virus (CaMV) 35S promoter and the beta-glucuronidase (gus) structural gene driven by a single copy of the CaMV 35S promoter between left- and right-border sequences. Herbicide-resistant, proliferating callus was obtained from explants on a medium containing both 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzyladenine (BA). |
1(0,0,0,1) | Details |