Protein Information

Name C18
Synonyms B1; D1; BBS 9; BBS9; BBS9 GENE; Bardet Biedl syndrome 9 protein; C18; PTH responsive osteosarcoma B1 protein…

Compound Information

Name sulfuric acid
CAS sulfuric acid

Reference List

PubMed Abstract RScore(About this table)
18969971 Wrobel K, Rodriguez DM, Aguilar FJ, Wrobel K: Determination of methanol in o,o-dimethyldithiophosphoric acid (DMDTPA) of technical grade by UV/vis spectrophotometry and by HPLC. Talanta. 2005 Mar 31;66(1):125-9. Epub 2004 Dec 8.

For chromatographic procedure, formaldehyde was derivatized with 2,4-dinitrophenylhydrazine and separation was achieved on Luna C18 (2) column using the isocratic elution with acetonitrile-water (70:30, v/v) and spectrophotometric detection at 360nm.
Free Pb (II) ions were eliminated with sulfuric acid and methanol was oxidized to formaldehyde with potassium permanganate in methanesulfonic acid medium.
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12548838 Chen X, Shi X: [Determination of monensin residue in chicken by HPLC with post-column derivatization]. Se Pu. 1999 Jan;17(1):77-9.

Monensin is separated on mu-Bondapak C18 column (3.9 mm i.d. x 300 mm) with methanol-water-phosphoric acid as a mobile phase and the flow rate was 0.7 mL/min.
The derivatization reagent consisted of 20 mL concentrated sulfuric acid, 950 mL methanol and 30 g vaniline.
1(0,0,0,1) Details
20176909 Gobin P, Lemaitre F, Marchand S, Couet W, Olivier JC: Assay of colistin and colistin methanesulfonate in plasma and urine by liquid chromatography tandem mass spectrometry (LC-MS/MS). Antimicrob Agents Chemother. 2010 Feb 22.

CMS concentrations were determined indirectly by substracting colistin concentrations determined in biological samples from the whole colistin concentrations determined after sample treatment with sulfuric acid in order to hydrolize CMS into colistin.
After extraction on SPE column, colistins were separated on an XBrigde (R) C18 column with isocratic elution (3.8 min runtime).
1(0,0,0,1) Details
12579983 Yang DL, Huang XN, Sun AS, Huang B, Ye L, Shi JS: [RP-HPLC method for determination of protopine in plasma and pharmacokinetics in rats]. Yao Xue Xue Bao. 2001 Oct;36(10):790-2.

METHODS: The column was packed with 5 microns C18.
After twice extracted with ether under basic condition, and reextracted with 0.02 mol.L-1 sulfuric acid, protopine in the plasma samples was isolated well.
1(0,0,0,1) Details
15638154 Li Y, Zhang J, Xiang R, Yang Y, Horvath C: Preparation and characterization of alkylated polymethacrylate monolithic columns for micro-HPLC of proteins. J Sep Sci. 2004 Dec;27(17-18):1467-74.

First, glycidyl groups at the surface of the porous monolith were hydrolyzed with sulfuric acid.
Separation performance for proteins on columns with C18 and C8 stationary phases was compared.
1(0,0,0,1) Details
16106862 Palmans R, Claes S, Vanatta LE, Coleman DE: Ion-pair chromatography of bis (sodium-sulfopropyl) disulfide brightener in acidic copper plating baths. J Chromatogr A. 2005 Aug 26;1085(1):147-54.

Addition of tetra-N-methylammonium cation as ion-pairing agent to a methanol-sulfuric acid-water eluent increases the retention time of the anionic SPS2- on a C18 column sufficiently to separate this compound from Cu (II) ion and additive by-products.
31(0,1,1,1) Details
17897656 Perez-Ruiz T, Martinez-Lozano C, Garcia MD: High-performance liquid chromatography-post-column chemiluminescence determination of aminopolycarboxylic acids at low concentration levels using tris (2,2'-bipyridyl) ruthenium (III). J Chromatogr A. 2007 Oct 26;1169(1-2):151-7. Epub 2007 Sep 14.

The aminopolycarboxylic acids were separated on a C18 reversed-phase column with an aqueous sulfuric acid mobile phase at a pH lower than 1.5.
6(0,0,1,1) Details
19007494 Morvan J, Hubert-Roux M, Agasse V, Cardinael P, Barbot F, Decock G, Bouillon JP: Separation and quantitative analysis of alkyl sulfate ethoxymers by HPLC. J Chromatogr Sci. 2008 Nov-Dec;46(10):872-82.


Separation of alkyl sulfate ethoxymers is investigated on various high-performance liquid chromatography (HPLC) stationary phases: Acclaim C18 Surfactant, Surfactant C8, and Hypercarb.
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17348702 Hsiao HH, Hsieh HY, Chou CC, Lin SY, Wang AH, Khoo KH: Concerted experimental approach for sequential mapping of peptides and phosphopeptides using C18-functionalized magnetic nanoparticles. J Proteome Res. 2007 Apr;6(4):1313-24. Epub 2007 Mar 10.

Upon binding, the initial aqueous wash allows desalting, while a second and a third wash with high acetonitrile content coupled with diluted sulfuric acid and ammonia removes most of the bound nonphosphorylated peptides.
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20153310 Gao W, Xie Q, Jin J, Qiao T, Wang H, Chen L, Deng H, Lu Z: HPLC-FLU detection of cortisol distribution in human hair. . Clin Biochem. 2010 Feb 10.

The extraction was derived with sulfuric acid.
The derivative was extracted with SPE C18 column.
1(0,0,0,1) Details
20149762 Hosoda K, Furuta T, Ishii K: Simultaneous determination of glucuronic acid and sulfuric acid conjugated metabolites of daidzein and genistein in human plasma by high-performance liquid chromatography. J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Mar 1;878(7-8):628-36. Epub 2010 Jan 25.

The HPLC assay was performed on a Hydrosphere C18 column (100 mm x 4.6 mm I.D., particle size 3 microm).
1(0,0,0,1) Details
16001838 Dousa M, Lemr K, Stransky Z: Determination of methylbenzoquate in premixes and animal feeds using liquid chromatography with fluorescence detection. J AOAC Int. 2005 May-Jun;88(3):673-8.

Both the final feed and premix extracts were analyzed by reversed-phase LC on a NovaPak C18 column (3.9 x 150 mm; 4 microm) with methanol-acetonitrile-water-phosphoric acid (340 + 350 + 308 + 2, v/v) as mobile phase.
Alternatively, post-column addition of sulfuric acid solution was used to decrease the determination limit.
1(0,0,0,1) Details