Protein Information

Name protein is
Synonyms ANX 2; p36; LIP 2; LIP2; ANX2; ANX2L4; ANX2P1; ANX2P2…

Compound Information

Name silica gel
CAS silica gel

Reference List

PubMed Abstract RScore(About this table)
16332093 Sottini S, Abbruzzetti S, Spyrakis F, Bettati S, Ronda L, Mozzarelli A, Viappiani C: Geminate rebinding in R-state hemoglobin: kinetic and computational evidence for multiple hydrophobic pockets. J Am Chem Soc. 2005 Dec 14;127(49):17427-32.

We show that the time course of CO rebinding to human hemoglobin also exhibits a biphasic geminate rebinding when the protein is entrapped in wet nanoporous silica gel.
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18447365 Shibayama N: Slow motion analysis of protein folding intermediates within wet silica gels. Biochemistry. 2008 May 27;47(21):5784-94. Epub 2008 May 1.

Resolving the complete folding pathway of a protein is a major challenge to conventional experimental methods because of the rapidity and complexity of folding.
Here, we show that entrapment of the protein cytochrome c in wet, optically transparent, porous silica gel matrices has enabled a dramatic expansion, to days or weeks, of the folding time, allowing direct observation of the entire folding pathway using a combination of three spectroscopic techniques, far-ultraviolet circular dichroism, tryptophan fluorescence, and Soret absorption spectroscopy.
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17429619 Cannone F, Collini M, Chirico G, Baldini G, Bettati S, Campanini B, Mozzarelli A: Environment effects on the oscillatory unfolding kinetics of GFP. Eur Biophys J. 2007 Sep;36(7):795-803. Epub 2007 Apr 12.

However, when close to unfolding, the A-N switchings suddenly become very regular as shown by fluorescence oscillations that have been recently observed for molecules embedded in wet silica gel.
In order to establish whether the matrix hosting the protein is responsible for these oscillations, we investigated the effect of another medium (silanized surfaces), of a different denaturant (urea) and of cosolvents (D (2) O and glycerol).
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