Protein Information

Name acid phosphatase
Synonyms ACP 3; PAP; ACP3; ACPP; ACPP protein; Acid phosphatase; Acid phosphatase prostate nirs variant 1; PACP…

Compound Information

Name 1-naphthol
CAS 1-naphthalenol

Reference List

PubMed Abstract RScore(About this table)
9584869 Luchter-Wasylewska E: Continuous assay for acid phosphatase using 1-naphthyl phosphate as a substrate. Acta Biochim Pol. 1997;44(4):853-9.

The described continuous acid phosphatase assay is based on kinetics of the release of 1-naphthol in the course of the enzyme-catalyzed hydrolysis of 1-naphthyl phosphate, measured at 320 nm in aqueous solution and at 322 nm in sodium-bis (2-ethylhexyl) sulfosuccinate isooctane-water reverse micelles in a broad pH range (1.0-8.2).
33(0,1,1,3) Details
7441169 Gundlach G, Muhlhausen B: [Coupling of 1-naphthol with fast-red-TR. J Clin Chem Clin Biochem. 1980 Oct;18(10):603-10.

In the course of studies on the optimization of a method for the continuous determination of acid phosphatase, the coupling of 1-naphthol with fast-red-TR was investigated: 1.
8(0,0,1,3) Details
7198943 Bowers JL, Bowers GN Jr: 1-naphthyl phosphate: specifications for high-quality substrate for measuring prostatic acid phosphatase activity. Clin Chem. 1982 Jan;28(1):212-5.

Four of the remaining eight lots of sodium 1-naphthyl phosphate met the following specifications: (a) sodium 1-naphthyl phosphate content greater than 80% by A286nm measurements (epsilon286nm1-NP = 5630 L.mol-1.cm-1) and by enzymic conversion to 1-naphthol (epsilon332nm1-N = 7560 L.mol-1.cm-1), (b) free 1-naphthol less than 3 mmol/mol, (c) inorganic phosphate less than 10 mmol/mol, and (d) the catalytic activity concentration greater than 98% maximum by absorbance assay and greater than 90% maximum by spectrofluorometric assay during simultaneous comparisons of several substrates.
2(0,0,0,2) Details