| 16549507 |
Bencsik AA, Coleman AW, Debeer SO, Perron H, Moussa A: Amplified immunohistochemical detection of PrPsc in animal transmissible spongiform encephalopathies using streptomycin. J Histochem Cytochem. 2006 Aug;54(8):849-53. Epub 2006 Mar 20.
Due to its sensitivity, immunohistochemistry (IHC) of abnormal prion protein (PrPsc) is used to study experimental and natural cases of transmissible spongiform encephalopathies (TSEs) such as Creutzfeldt-Jakob disease in humans or scrapie and bovine spongiform encephalopathy (BSE) in animals. The limits of detection are particularly critical when PrPsc IHC is used for diagnostic purposes. In this article, we describe for the first time the use of streptomycin sulfate in IHC, providing a novel original and easy way to amplify specifically PrPsc immunohistochemical detection in natural cases of BSE and scrapie, as well as in experimental TSEs in mice models using two different PrP antibodies. |
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