Protein Information

ID 1065
Name phosphoglycerate kinase
Synonyms MIG10; PGK 1; PGK1; PGKA; PRP 2; Phosphoglycerate kinase; Phosphoglycerate kinase 1; Primer recognition protein 2…

Compound Information

ID 366
Name anthraquinone
CAS 9,10-anthracenedione

Reference

PubMed Abstract RScore(About this table)
376530 Beissner RS, Rudolph FB: Effect of anthraquinone dyes and evaluation of the kinetic mechanism of yeast phosphoglycerate kinase. J Biol Chem. 1979 Jul 25;254(14):6273-7.
Cibacron Blue 3G-A (CB3G-A, I) was investigated as a ternary complex analogue of lactate dehydrogenase and phosphoglycerate kinase as had been suggested earlier (Stellwagen, E. (1977) Accts. Chem. Res. 10, 92-98). CB3G-A and Procion Brilliant Blue (PBB, II), a structural isomer of the Cibacron dye without the sulfonated benzoyl moiety, were attached covalently to Sepharose CL-6B. The two enzymes were adsorbed to the columns and then eluted by substrates in various combinations. CB3G-A and PBB interact similarly with the two enzymes in spite of the structural differences between the dyes indicating that the specific structure of CB3G-A is not a ternary complex analogue. Inhibition studies of phosphoglycerate kinase by CB3G-A suggest that 2 molecules of dye bind per monomer and are consistent with multiple substrate binding sites. It is suggested that the kinetic mechanism of phosphoglycerate kinase is best described as steady state random.
84(1,1,1,4)