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Feng F, Uno B, Goto M, Zhang ZX, An DK: Evaluation of anthraquinone-2-sulfonyl chloride for determination of phenol in water by liquid chromatography using pre-column phase-transfer catalysed derivatization. Phytochem Anal. 2008 Mar;19(2):122-6.
A new reagent, anthraquinone-2-sulfonyl chloride (ASC), has been used for the derivatization of phenols. Several compounds with different polarity were selected to evaluate the new reagent and derivatives of these phenols prepared via a facile pathway. The optimal conditions for analytical derivatization and mechanism of the derivatization reaction are discussed. The derivatization procedure involves an ion-pair extraction of the deprotonated phenols with a tetrabutylammonium counter ion to an organic phase. At the interface of two phases, the derivatization reaction occurs quantitatively at room temperature within 3 min. The derivatives are stable and readily amenable to analysis by normal-phase and reversed-phase high performance liquid chromatography (HPLC). Excellent linearity response was demonstrated over the concentration range of 0.2 mumol/L to 200 mumol/L at 320 nm for normal-phase HPLC, at 256 nm for reversed-phase HPLC. Combined with preconcentration using a Waters Sep-Pak Plus C18 cartridge, detection limits of phenols for water sample analysis were as low as 1 x 10 (-9) mol/L (ca. 0.1 microgram/L). |
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