| 12851734 |
Perez-Ruiz T, Martinez-Lozano C, Tomas V, Martin J: Determination of ATP via the photochemical generation of hydrogen peroxide using flow injection luminol chemiluminescence detection. Anal Bioanal Chem. 2003 Sep;377(1):189-94. Epub 2003 Jul 8.
The determination of ATP using the coupling between a photochemical reaction and a chemiluminescence reaction in a flow injection (FI) system is described. The method is based on the reaction of glucose with ATP catalyzed by hexokinase and Mg (2+) ions. The glucose that is not consumed by ATP is subsequently photooxidized using 9,10-anthraquinone-2,6-disulfonate as a sensitizer. The hydrogen peroxide produced in the photochemical reaction is monitored through the chemiluminescence reaction with luminol catalyzed by hematine. There is a linear relationship between the decrease in the chemiluminescence response and the ATP concentration at a constant concentration of glucose. Under the optimum conditions, the calibration graph is linear in the range 0.20-50.5 mg L (-1) with a throughput of 25 samples per hour and relative standard deviations between +/-0.62 and +/-1.42%. The limit of detection is 0.07 mg L (-1). The method was used for the determination of ATP in pharmaceuticals, milk, and soils. |
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