| 6675378 |
Ohlsson PI, Paul KG: The reduction potential of lactoperoxidase. . Acta Chem Scand B. 1983;37(10):917-21.
The reduction potential of Fe (III)/Fe (II) lactoperoxidase has been determined. Optical determinations of equilibria with 2-methyl-3-hydroxy-1,4-naphthoquinone as indicator and prereduced 9,10-anthraquinone-2-sulfonate as reducing agent gave Em,7.0 = -191 +/- 2 mV. Potentiometric determinations with 9,10-anthraquinone-2-sulfonate as mediator and, in the reduced form as reducing agent, gave Em,7.0 = -188 +/- 1 mV. Addition of 0.5% N-cetyl-N,N,N-trimethyl-ammonium bromide, and using dithionite as reducing agent, gave Em,7.0 = -183 mV and -179 mV with 9,10-anthraquinone-2-sulfonate and 9,10-anthraquinone-2,6-disulfonate, respectively, as mediators. |
2(0,0,0,2) |