| 14577168 |
Slinchenko NM, Chernysh IH, Kosterin SO: [Utilization of purified myometrium cell plasma membrane Ca2+, Mg2+-ATPase for comparative estimation of the efficiency of energy-dependent Ca2+-transport inhibitors]. Ukr Biokhim Zh. 2003 Mar-Apr;75(2):33-8.
With the aim of comparative estimation of efficacy of well-known inhibitors of energy-dependent Ca (2+)-transporting systems their effects were investigated on the activity of purified Ca2+, Mg (2+)-ATPase of the myometrium cell plasma membranes. From the approved inhibitors (eosin Y, o-vanadate, thapsigargin, cyclopiazonic acid, ruthenium red, sodium azide) only eosin Y and o-vanadate are potent inhibitors of myometrium sarcolemma Ca (2+)-pump: the values of Ki equal 0.8 and 4.7 microM, respectively. Thapsigargin and cyclopiazonic acid as well as ruthenium red in concentrations inhibiting, respectively, endo (sarco) plasmic reticulum Ca (2+)-pump and energy-dependent Ca (2+)-transport in mitochondria had no effect on the Ca2+, Mg (2+)-ATPase of the uterus smooth muscle cell plasma membrane. Sodium azide (10 mM) blocking completely Ca (2+)-transport in mitochondria inhibited activity of the plasma membrane Ca (2+)-transporting ATPase by 14%. |
83(1,1,1,3) |