| 2156358 |
Chetty CS, Rajanna B, Rajanna S: Inhibition of rat brain microsomal Na+/K (+)-ATPase and ouabain binding by mercuric chloride. Toxicol Lett. 1990 Mar;51(1):109-16.
This study concerned the effects of mercuric chloride on Na+/K (+)-ATPase and [3H] ouabain binding in rat brain microsomes in vitro. The data showed that HgCl2 inhibited Na+/K (+)-ATPase effectively at micromolar concentrations. The degree of inhibition was decreased with increases in enzyme concentration and incubation time. Variations in the ionic strength of Na+ and K+ did not alter the percent inhibition of Na+/K (+)-ATPase activity by HgCl2. Repeated washings partially restored enzyme activity. The binding of [3H] ouabain to microsomal membranes was inhibited by HgCl2 in a concentration-dependent manner. Cumulative inhibition studies with HgCl2 and ouabain indicated that these inhibitors did not act concurrently and independently on Na+/K (+)-ATPase. |
113(1,2,2,3) |