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Aluigi MG, Guida C, Falugi C: Apoptosis as a specific biomarker of diazinon toxicity in NTera2-D1 cells. Chem Biol Interact. 2010 Mar 22.
The NTera2/D1 (NT2) cell line, which was derived from a human teratocarcinoma, exhibits properties that are characteristic of a committed neuronal precursor at an early stage of differentiation. Its property to express a whole set of molecules related to the cholinergic neurotransmission system, including active acetylcholinesterase (AChE, EC 3.1.1.7) makes it a good alternative model for testing the effects of neurotoxic compounds, such as organophosphorus (OP) insecticides, whose primary target is the inhibition of AChE activity. Recent findings have elucidated the role of AChE in modulation of apoptosis, but the mechanisms are still rather obscure. NT2 cells exposed to the OP insecticide diazinon at concentrations ranging between 10 (-4)-10 (-5) M showed a time-dependent enhancement of cell death. When exposed at 10 (-6) M diazinon, showed higher cell viability than control samples up to 72h, followed by a decreasing phase. The cell death caused by the exposures showed a number of features characteristic of apoptosis, including membrane and mitochondrial potential changes. We suggest the hypothesis that such a behaviour is due to a dynamic balance between activated and blocked acetylcholine receptors, that in turn trigger electrical events and caspases cascade. |
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