| 19111380 |
Sood N, Lal B: Isolation of a novel yeast strain Candida digboiensis TERI ASN6 capable of degrading petroleum hydrocarbons in acidic conditions. J Environ Manage. 2009 Apr;90(5):1728-36. Epub 2008 Dec 25.
A novel yeast species Candida digboiensis TERI ASN6 was isolated from soil samples contaminated with acidic oily sludge (pH 1-3) from the Digboi refinery (Northeast India). The strain TERI ASN6 could degrade 73% of the total petroleum hydrocarbons present in the medium at pH 3 in a week. This strain presents a dimorphic behaviour and showed mycelia morphology when grown under stressed conditions such as low pH and in a medium containing petroleum hydrocarbons. The C. digboiensis strain could efficiently degrade the aliphatic and aromatic fractions of the acidic oily sludge at pH 3 as confirmed by gas chromatography. During the growth of TERI ASN6 in dibenzothiophene (DBT), DBT-sulfone and biphenyl-2-ol were detected. An active cytochrome P450 system, implicated in hydrocarbon oxidation, was also detected in this yeast using degenerated primers based on its conserved regions. This yeast is a potential candidate for petroleum bioremediation treatment of hydrocarbon contaminated acidic soils. Its physiological behaviour allows the strain to work efficiently where other hydrocarbon-degrading bacteria may not survive. |
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