| 19117374 |
Carlson EA, Roy NK, Wirgin II: Microarray Analysis of Polychlorinated Biphenyl Mixture-Induced Changes in Gene Expression among Atlantic Tomcod Populations Displaying Differential Sensitivity to Halogenated Aromatic Hydrocarbons. Environ Toxicol Chem. 2008 Dec 31:1.
Several populations of fishes inhabiting contaminated Atlantic Coast estuaries exhibit resistance to early life-stage (ELS) toxicities induced by halogenated aromatic hydrocarbons such as coplanar polychlorinated biphenyls (PCBs). These toxicities include mortality, circulatory failure, edema, and craniofacial malformations. The mechanisms behind resistance to HAH toxicity in these populations are unknown. First and second generation Atlantic tomcod Microgadus tomcod embryos derived from the Hudson River (HR; NY, USA) population are highly resistant to PCB -induced cytochrome P450 1A (CYP1A) expression and ELS toxicity when compared to embryos of Miramichi River (MR; NB, Canada) and Shinnecock Bay (SB; NY, USA) origin. The present study sought to identify novel genes involved in population differences in response to PCB exposure using custom microarrays. Microarray probes consisted of un-sequenced inserts of randomly-picked clones from a tomcod cardiac cDNA library. Tomcod embryos from three populations (HR, MR, and SB) were exposed to two doses of an environmentally-relevant mixture of coplanar PCBs and screened for dose- and population-specific patterns of gene expression. Clones displaying significant differences between populations exposed to the high dose of PCBs were identified by DNA sequencing. Of the 28 identified non-ribosomal protein clones, none displayed expression patterns highly similar to CYP1A (altered in MR and SB, but not in HR). However, several transcripts representing biomarkers of cardiomyopathy in mammals (cardiac troponin T2, cathepsin L, and atrial natriuretic peptide) were differentially altered among the 3 tomcod populations by PCBs. Although the present study did not identify any novel genes associated with PCB resistance in tomcod, several potential molecular biomarkers of PCB exposure were revealed. |
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