| 2463365 |
Schmid A, Gogelein H, Kemmer TP, Schulz I: Anion channels in giant liposomes made of endoplasmic reticulum vesicles from rat exocrine pancreas. J Membr Biol. 1988 Sep;104(3):275-82.
Using the method of dehydration and rehydration, rough endoplasmic reticulum (RER) vesicles, isolated by differential centrifugation, can be enlarged to giant liposomes with diameters ranging from 5 to 200 micron. Patch-clamp studies on these giant RER liposomes revealed the existence of a channel with a mean conductance of 260 +/- 7 pS (n = 23; 140 mmol/liter KCl on both sides). The channel is about four times more permeable for Cl- than for K+. Its activity is strongly voltage regulated. At low potentials (+/- 20 mV) the channel is predominantly in its open state with an open probability near 1.0, whereas it closes permanently at high positive and negative voltages (+/- 70 mV). The channel activity is not influenced by changing the free Ca2+ concentration from 1 mmol/liter to less than 10 (-9) mol/liter on either side, and is also not affected by typical Cl- -channel blockers like diphenylamine-2-carboxylate (DPC, 1 mmol/liter) or 4-acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic acid (SITS, 1 mmol/liter). Another chloride channel with a single-channel conductance of 79 +/- 6 pS (n = 4) was less frequently observed. In the potential range of -80 to +40 mV this channel displayed no voltage-dependent gating. We assume that these anion channels are involved in the maintenance of electroneutrality during Ca2+ uptake in the RER. |
1(0,0,0,1) |