| 3208630 |
Shuman J, Przybyla A: Expression of the 31-kD stress protein in rat myoblasts and hepatocytes. . DNA. 1988 Sep;7(7):475-82.
Rat tissue cultures cells respond to stress by inducing the synthesis of about 20 proteins, including two low-molecular-weight species of about 31 kD and 27 kD. We have cloned a cDNA for the 31-kD protein. This protein is induced in myoblasts and hepatoma cells in response to a 43 degrees C heat shock, or exposure to sodium arsenite or cadmium chloride salts. Furthermore, this protein is superinduced in hepatoma cells conditioned to grow in cadmium and zinc salts when they are exposed to a standard sodium arsenite stress. Induction of the gene encoding the 31-kD protein has been characterized as follows: (i) Transcripts accumulate maximally with similar kinetics when myoblasts are induced with either heat shock or sodium arsenite; (ii) accumulation of transcripts decays to preinduction levels within 4 hr of a heat shock, but requires more than 8 days after sodium arsenite stress; (iii) basal levels of transcript are reduced when myoblasts are cultured in the presence of steroid hormones; and (iv) stress induction is virtually abolished once myoblasts have differentiated. |
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