| 7082482 |
Testai E, Citti L, Gervasi PG, Turchi G: [In vitro hepatic cytochrome P-450 destruction induced by 1,2-dichloroethylene]. Boll Soc Ital Biol Sper. 1982 May 15;58(9):513-9.
An estimation of the rate of 1,2-DCE metabolism by the hepatic microsomal cytochrome P-450 system was obtained by monitoring the oxidation of NADPH at 340 nm. During such a metabolism, cytochrome P-450 from PB-pretreated male mice is destroyed in vitro in presence of NADPH, after 30 minutes of incubation at 37 degrees C by 1,2-DCE. The NADPH-request suggests that oxidative metabolism of this olefin by cytochrome P-450 is necessary for the observed destruction. The loss of cyt.P-450 could be attributed to heme destruction, as the destructive interaction is always accompanied by a correlated loss of microsomal heme. |
4(0,0,0,4) |