| 17022664 |
Wiwatchaiwong S, Nakamura N, Ohno H: Spectroscopic characterization and electrochemistry of poly (ethylene oxide)-modified myoglobin in organic solvents. Biotechnol Prog. 2006 Sep-Oct;22(5):1276-81.
Myoglobin (Mb) was chemically modified with activated poly (ethylene oxide) (PEO) (average molecular weight of 2000) to solubilize it in various organic solvents. UV-vis, circular dichroism, and Raman spectroscopy were used to characterize the structure correlated with the electron-transfer reactions of PEO-modified Mb (PEO-Mb). Spectroscopic data indicated changes in heme coordination geometry for PEO-Mb in various organic solvents that are different from that in water. The Raman spectrum showed the characteristics of PEO-Mb in PEO oligomer (average MW of 200) in the five-coordinate high-spin state, which facilitates fast electron-transfer reactions between protein and the glassy carbon electrode. These results suggest heme environment effects on the properties of proteins in organic solvents. |
162(2,2,2,2) |