46656 |
Tulchin N, Ornstein L, Weiner P: C3, GBG, orosomucoid and haptoglobin polymorphisms. Vox Sang. 1975;28(1):74-6. Improved staining methods.. C3, GBG, and orosomucoid polymorphisms were electrophoresed in a high-voltage agarose method which permitted the typing of 15-20 samples. The increased sensitivity of the dye Coommassie blue was used to stain the protein, and yielded higher resolution than amido black. The typing of haptoglobin samples, was facilitated by devising a method which utilizes the peroxidase activity of the haptoglobin-hemoglobin complex with 90-tolidine and hydrogen peroxide as substrates and 4-chloro-1-naphthol as coupler. |
82(1,1,1,2) |