| 7581838 |
Zihnioglu F, Telefoncu A: Substrate specificity and the use of chitosan-entrapped rabbit hepatic microsomal UDP-glucuronyl transferase for detoxification. Artif Cells Blood Substit Immobil Biotechnol. 1995;23(4):533-43.
Rabbit hepatic microsomal UDP-Glucuronyl transferase (EC.2.4.1.17) was immobilized in chitosan, which is an ionotropic gelatation agent, and the resulting preparation was used as a biocatalyst for the glucuronidation of toxic substances and drugs such as; 1-naphthol, phenol, 4-nitrophenol, bilirubin, testesterone, estrone, lamotrigine, imipramine and chlorpromazine. For studying the drug metabolism in vitro and obtaining artificial liver support (detoxification) chitosan-entrapped UDPGT towards 1-naphthol was tested by using two model reactor system. The conversion curves of 1-naphthol to its glucuronide indicated that the column reactor system was found to be better and seems suitable for detoxification with a high yield of glucuronide formation. |
32(0,1,1,2) |