| 16439107 |
Wu Z, Wu J, Wang S, Shen G, Yu R: An amplified mass piezoelectric immunosensor for Schistosoma japonicum. . Biosens Bioelectron. 2006 Aug 15;22(2):207-12. Epub 2006 Jan 24.
An ultrasensitive piezoelectric immunosensor using an amplification path based on an insoluble biocatalyzed precipitation product has proposed for Schistosoma japonicum. A mercapto Schistosoma japonicum antigen was self-assembled onto the quartz crystal surface via an Au nanoparticle mediator monolayer to sense the Schistosoma japonicum antibody (SjAb). And the horseradish peroxidase labeled protein A conjugate which was bounded to the SjAb by a "sandwich" format was used as a biocatalyst for the oxidative precipitation of 4-chloro-1-naphthol by H (2) O (2) to yield the insoluble product benzo-4-chlorohexadienone, resulting in an amplified mass sensing of antigen-antibody interaction. The amount of the precipitate accumulated on the quartz crystal is controlled by the antibody concentration. The SjAb can be linearly determined in the range of 10-200 ngml (-1) and the detection limit reaches as low as 5 ngml (-1). |
31(0,1,1,1) |