| 6625179 |
Esen A, Conroy JM, Wang SZ: A simple and rapid dot-immunobinding assay for zein and other prolamins. Anal Biochem. 1983 Jul 15;132(2):462-7.
A simple method for the immunochemical assay of zein and other prolamins is described. Alcohol-solubilized zein is spotted onto chromatography paper disks or strips, incubated with antiserum (30-60 min), washed with several changes of Tris-buffered saline (10 min), incubated with protein A-peroxidase (30 min), and washed with several changes of Tris-buffered saline (10 min). the binding of zein-specific antibody is visualized by peroxidase-catalyzed color production from 4-chloro-1-naphthol. The method is simple, inexpensive, and rapid (2 h total time) and has potential application to other insoluble antigens. |
31(0,1,1,1) |