| 3579898 |
Long AR, Chism GW 3rd: The effect of metyrapone on cytokinin ([8-14C] benzylaminopurine) metabolism in mature green tomato pericarp. Biochem Biophys Res Commun. 1987 Apr 14;144(1):109-14.
Cytokinin, [8-14C] Benzylaminopurine, metabolism in tomato pericarp was followed during a 3 h period utilizing thin layer chromatography and visualization by fluorography. Fluorography indicated the formation of at least 7 metabolites during 3 h. Cytokinin metabolism was reduced by approximately 40% in 3 h by the presence of 250 microM metyrapone, an inhibitor of cytochrome P-450 related enzyme systems. In the presence of metyrapone, the number of radioactive metabolites on the thin layer plate was reduced from 7 to 4 and 2 of these were unique to the metyrapone-treated sample. These data suggest the initial step in benzylaminopurine metabolism in tomato pericarp may be mediated by a cytochrome P-450 related enzyme system which is altered in the presence of metyrapone. |
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