| 14514073 |
Chen SA, Wang X, Zhao B, Yuan X, Wang Y: Production of crocin using Crocus sativus callus by two-stage culture system. Biotechnol Lett. 2003 Aug;25(15):1235-8.
Saffron callus was grown in a two-stage culture on B5 medium supplemented with casein hydrolysate (300 mg l (-1)) at 22 degrees C in dark with naphthalene acetic acid (2 mg l (-1)) and 6-benzyladenine (1 mg l (-1)) to give maximum biomass (16 g dry wt l (-1)), and with indole 3-acetic acid (2 mg l (-1)) and 6-benzyladenine (0.5 mg l (-1)) for crocin formation. The maximum crocin production (0.43 g l (-1)) was achieved by this two-stage culture method, which was three times that by a one-stage method. |
6(0,0,1,1) |