| 16011277 |
Zhang AM, Yang SY, Xue JP, Sheng W, Gao X, Zhu YF: [Effects of different factors on direct induction of microtubers from explants in Pinellia ternate]. Zhongguo Zhong Yao Za Zhi. 2005 Apr;30(8):576-9.
OBJECTIVE: To study the effects of different factors on direct induction of microtubers. These factors included plant growth substances, casein hydrolysate (CH), active carbon (AC), polyethylene glycol (PEG 4000), sucrose and glucose. METHOD: Using the orthogonal design method. RESULT AND CONCLUSION: The optimal media to directly induce microtubers from leaves were MS + MET 0.5 mg x L (-1) + 6-BA 0.5 mg x L (-1) + sucrose 3% and MS+ 6-BA 0.5 mg x L (-1) + IAA 0.5 mg x L (-1) + sucrose 3%. Optimal media to directly induce microtubers from tubers were MS + 6-BA 1.0 mg x L (-1) + PEG 5% + sucrose 5% and MS+ CH 500 mg x L (-1) + MET 0.5 mg x L (-1) + 6-BA 0.5 mg x L (-1) + AC 0.5% + sucrose 5%. Media suitable for plantlet growth were MS + 6-BA 0.5 mg x L (-1) + IAA 0.5 mg x L (-1) + AC 0.5% + sucrose 5% and MS + MET 0.5 mg x L (-1) + 6-BA 0.5 mg x L (-1) + sucrose 3%. |
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