| 20138169 |
Son JH, Heo YJ, Park MY, Kim HH, Lee KS: Optimization of cryopreservation condition for hematopoietic stem cells from umbilical cord blood. Cryobiology. 2010 Feb 4.
The conditions for cryopreservation of CD34 (+) hematopoietic stem cells (HSC) from umbilical cord blood (UCB) were optimized with a new cryo-medium containing 10% ethylene glycol (EG) and 2% dimethyl sulfoxide (Me (2) SO) using a controlled-rate freezing (CRF) method. After the cryopreservation of mononuclear cells (MNC) from UCB, recoveries of MNC, CD34 (+) cells, and total colony-forming units (CFU) were significantly improved compared to those in the control cryo-medium containing 10% Me (2) SO and 2% Dextran-40 (P <0.05). This study shows that the new cryo-medium and CRF method provide better recoveries of MNC, HSC and total CFU than the control cryo-medium and isopropylalcohol freezing (IPA) method. Therefore, this cryo-medium, combined with the CRF method, is valuable for optimizing cryopreservation conditions for HSC from UCB to obtain satisfactory HSC recovery. |
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