| 10691637 |
Alcocer MJ, Doyen C, Lee HA, Morgan MR: Functional scFv antibody sequences against the organophosphorus pesticide chlorpyrifos. J Agric Food Chem. 2000 Feb;48(2):335-7.
Two functional single-chain Fv (scFv) antibodies that recognize specifically the widely used organophosphorus pesticide chlorpyrifos-ethyl were derived from two murine hybridoma cell lines. It is shown that the functional scFvs could be isolated without any rounds of selection, with a success rate dependent on the efficiency of amplification of the functional light chain gene family by the specific primers. Besides four new functional immunoglobulin variable gene sequences, the isolation of a new pseudogene is reported. |
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