Protein Information

ID 95
Name cholinesterase
Synonyms Acylcholine acylhydrolase; BCHE; BCHE protein; Butyrylcholine esterase; Butyrylcholinesterase; CHE1; Choline esterase II; Cholinesterase…

Compound Information

ID 202
Name chlorpyrifos
CAS

Reference

PubMed Abstract RScore(About this table)
19575182 Noort D, Hulst AG, van Zuylen A, van Rijssel E, van der Schans MJ: Covalent binding of organophosphorothioates to albumin: a new perspective for OP-pesticide biomonitoring?. Arch Toxicol. 2009 Nov;83(11):1031-6. Epub 2009 Jul 4.
We here report on the covalent binding of various organophosphorothioate (OPT) pesticides to albumin at in vitro exposure levels that did not give rise to butyrylcholinesterase inhibition. Adduct formation occurred at the Tyr-411 residue of albumin, as was firmly corroborated by LC-tandem MS analysis of a pepsin digest of OPT-modified albumin. It cannot be excluded that other (tyrosine) residues become modified as well. A convenient method for mass spectrometric determination of the OPT tyrosine adduct has also been developed based on the pronase digestion of albumin and subsequent LC-tandem MS analysis of the digest. The resulting tyrosine phosphorothioate ester displayed favorable chromatographic and mass spectrometric properties for sensitive analysis. In vitro exposure levels of parathion and chlorpyrifos down to 1 microM could readily be assessed. The remarkable affinity of OPTs for albumin opens the way for a more complete assessment of OP pesticide exposure.
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