| 15802843 |
Thetkathuek A, Keifer M, Fungladda W, Kaewkungwal J, Padungtod C, Wilson B, Mankhetkorn S: Spectrophotometric determination of plasma and red blood cell cholinesterase activity of 53 fruit farm workers pre- and post-exposed chlorpyrifos for one fruit crop. Chem Pharm Bull. 2005 Apr;53(4):422-4.
We sought to investigate the early biological effects of chlorpyrifos among 53 Thai fruit farm workers by measuring the plasma cholinesterase (PChE) and red blood cell cholinesterase (AChE) activities, a biomarker of organophosphate (OPs) pesticide during one fruit crop. The ChE activity (V (m)/K (m)) was spectrophotometrically analyzed before and after exposing to chlorpyrifos. The V (m)/K (m) values of both non-spraying and spraying seasons are found as normal distribution pattern. The median PChE and AChE activities among farm workers in the non-spraying season were 2.3 x 10 (-3) s (-1) and 7.26 x 10 (-5) s (-1), respectively. The median PChE and AChE activities of the farm workers in the spraying season were 2.02 x 10 (-3) s (-1) and 5.95 x 10 (-5) s (-1), respectively. The mean V (m)/K (m) values of PChE shifted left (t-test, p=0.013), indicating a decrease in PChE activity in the farm workers exposed to chlorpyrifos. However, the V (m)/K (m) values of AChE in nonspraying season and in the spraying season were not different (t-test, p=0.246). We propose that PChE activity can be used as a biomarker for monitoring early toxicity induced by chlorpyrifos insecticide. |
8(0,0,1,3) |