| 10998265 |
Charpentier A, Menozzi P, Marcel V, Villatte F, Fournier D: A method to estimate acetylcholinesterase-active sites and turnover in insects. Anal Biochem. 2000 Oct 1;285(1):76-81.
Acetylcholinesterase is the primary target of organophosphorous and carbamate insecticides. Quantitative changes in acetylcholinesterase are suspected to confer resistance to these insecticides, but a method to estimate the amount in insect is not available. A method using irreversible inhibitors has been developed. Among the irreversible inhibitors tested, 7-(methylethoxyphosphinyloxy)-1-methylquinolinium iodide, chlorpyrifos-ethyl-oxon, and coumaphos-oxon were found to be sufficiently potent and specific. |
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