| 18595403 |
Zhang Z, Hu J: Effects of p,p'-DDE exposure on gonadal development and gene expression in Japanese medaka (Oryzias latipes). J Environ Sci. 2008;20(3):347-52.
Although 1,1-dichloro-2,2-bis (p-chlorophenyl)-ethylene (p,p'-DDE), the major and most persistent metabolite of dichlorodiphenyltrichloroethane (DDT), was continually detected in wild fishes that showed abnormal gonad development such as intersex, little is known about the impact of p,p'-DDE exposure on gonad development in fishes. To survey the effects of p,p'-DDE on gonadal development and gene expressions, male juvenile (20-d post hatch) Japanese medaka (Oryzias latipes) was exposed to 1, 5, 20, and 100 microg/L p,p'-DDE for two months. Increased hepatosomatic index (HSI) and decreased gonadosomatic index (GSI) were found in the p,p'-DDE-treated groups. Intersex was found in 100 microg/L p,p'-DDE exposure group, as well as 100 ng/L 17alpha-ethynylestradiol (EE2) group. By quantitative real-time RT-PCR, it was found that gene expressions of vitellogenins (VTG-1, VTG-2), choriogenins (CHG-H, CHG-L), and estrogen receptor alpha (ER-alpha) in the liver of the fish were significantly up-regulated byp,p'-DDE exposure. VTG-1 and VTG-2 were recommended as the preferred biomarker for assessing anti-androgenic p,p'-DDE because they were the highest up-regulated among the genes and showed good dose-response relationship. The up-regulated ER-alpha suggested that a potential synergetic effect would occur when p,p'-DDE coexists with other ER-alpha-binding endocrine-disrupting chemicals (EDCs). |
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