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Gobel E, Riessner R, Pohl P: [Influence of DCMU on the formation of lipids and fatty acids, and on the ultrastructure of Euglena gracilis (author's transl)]. Z Naturforsch C. 1976 Nov-Dec;31(11-12):687-92.
Euglena gracilis Klebs (strain Z) was grown heterotrophically in the dark for 6 days. Subsequently, the cells were grown photoautotrophically under white fluorescent light with varying amounts of 3-(3,4-dichlorophenyl)-1,1 dimethyl urea (DCMU) (1.7X10 (-9)--1.0X10 (-5) mol/l) added to the nutrient medium. Under non-photosynthetic conditions (addition of 10 (-6)--10 (-5) mol DCMU/l), the cells still produced chloroplast structures and a considerable percentage of compounds typical for chloroplasts, such as galactolipids (40-50%), chlorophylls (40%), and polyunsaturated C16 and C18 fatty acids (16:2, 16:3, 16:4, 18:2, 18:3) (65%). The results indicate that under these conditions the above compounds are synthesized from carbon sources outside the chloroplasts. |
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