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Varsano R, Matringe M, Magnin N, Mornet R, Scalla R: Competitive interaction of three peroxidizing herbicides with the binding of [3H] acifluorfen to corn etioplast membranes. FEBS Lett. 1990 Oct 15;272(1-2):106-8.
The specific binding of the herbicide acifluorfen 5-[2-chloro-4-(trifluoromethyl) phenoxy]-2-nitrobenzoic acid to corn etioplast membranes is competitively inhibited by protoporphyrinogen IX, the substrate of protoporphyrinogen oxidase. Three other peroxidizing molecules, oxadiazon [5-terbutyl-3-(2,4-dichloro-5-isopropoxyphenyl)-1,3,4-oxadiazol -2-one], LS 82556 [(S) 3-N-(methylbenzyl) carbamoyl-5-propionyl-2,6-lutidine], and M&B; 39279 [5-amino-4-cyano-1-(2,6-dichloro-4-trifluoromethylphenyl) pyrazol], also compete with acifluorfen for its binding site. The four herbicides thus bind to the same site, or to closely located sites, on the enzyme protoporphyrinogen oxidase. |
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