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Zhao HY, Xu XX, Zhang JX, Zheng W, Zheng YF: Carbon nanotube-hydroxyapatite-hemoglobin nanocomposites with high bioelectrocatalytic activity. Bioelectrochemistry. 2010 Jun;78(2):124-9. Epub 2009 Sep 6.
In this paper, carbon nanotubes-hydroxyapatite (MWCNTs-HA) composite were synthesized by self-assembling technique, and investigated by X-ray diffraction, scanning electron microscopy (SEM) and FT-IR spectroscopy. Hemoglobin (Hb) immobilized in MWCNTs-HA film not only retained its similarly native conformations, but also achieved the direct electron transfer. The apparent heterogeneous electron transfer rate constant (k (s)) was evaluated as (5.05+/-0.41) s (-1) according to Laviron's equation, and the surface coverage (Gamma*) was estimated as (9.25+/-0.69) x10 (-10) mol cm (-2). Moreover, the Hb immobilized in MWCNTs-HA film exhibited remarkable bioelectrocatalytic activity for the electrochemical reduction of H (2) O (2). The amperometric response of the biosensor varied linearly with the H (2) O (2) concentration ranging from 0.5microM to 2microM, and the results showed a detection limit of 0.09microM. Furthermore, the Hb-immobilized MWCNTs-HA film exhibited the excellent catalytic reactivity toward trichloroacetic acid (TCA). |
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