| 12168034 |
Cheng A, Wu MP, Lou B, Chen PF, Yang XQ, Mei MZ: Metabolism of Oxidized High Density Lipoprotein-2 (ox-HDL (2)) in Rat Hepatic Sinusoidal Cells. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao. 1998;30(4):343-346.
Incubation of rat hepatic sinusoidal cells with FITC-HDL (2), FITC-ox-HDL (2) and [(3) H] CE-HDL (2)(rHDL (2)), ox-rHDL (2) showed that binding of FITC-HDL (2) to the cells was competitive to ox-HDL (2), but not to HDL (2). The cell-endocytic fluorescence strength (FS) of FITC-HDL (2) and radioactivity of ox-rHDL (2) were 45.5% of that of FITC-HDL (2) and 61.4% of that of rHDL (2), respectively. Endocytic FS and radioactivity were mainly in TCA-precipitable and supernatant part, respectively. The cell-released FS and radioactivity were 67.7% and 10.9% of the cell-endocytic FS and the radioactivity, respectively, and both of them were mainly TCA-precipitable. These results suggest that: (1) There is probably an ox-HDL receptor on the surface of rat hepatic sinusoidal cells, which is different from HDL receptor. (2) The metabolic behaviour of ox-rHDL (2) in the cells is similar to HDL (2). Both of them do not take a lysosomal pathway. Apoproteins and CE components dissociate from endocytic lipoprotein in the cells. After the cells have taken up most of CE, the residual CE recombines with apolipoprotein to form a lipoprotein and is released from the cells by retroendocytosis. (3) Oxidative modification of HDL (2) weakens its ability to cholesterol reverse transport. |
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