| 18836689 |
Zhao Q, Zhao YJ, Zhao BC, Ge RC, Li M, Shen YZ, Huang ZJ: Cloning and functional analysis of wheat V-H+-ATPase subunit genes. Plant Mol Biol. 2009 Jan;69(1-2):33-46. Epub 2008 Oct 4.
The root microsomal proteomes of salt-tolerant and salt-sensitive wheat lines under salt stress were analyzed by two-dimensional electrophoresis and mass spectrum. A wheat V-H (+)-ATPase E subunit protein was obtained whose expression was enhanced by salt stress. In silicon cloning identified the full-length cDNA sequences of nine subunits and partial cDNA sequences of two subunits of wheat V-H (+)-ATPase. The expression profiles of these V-H (+)-ATPase subunits in roots and leaves of both salt-tolerant and salt-sensitive wheat lines under salt and abscisic acid (ABA) stress were analyzed. The results indicate that the coordinated enhancement of the expression of V-H (+)-ATPase subunits under salt and ABA stress is an important factor determining improved salt tolerance in wheat. The expression of these subunits was tissue-specific. Overexpression of the E subunit by transgenic Arabidopsis thaliana was able to enhance seed germination, root growth and adult seedling growth under salt stress. |
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