| 8284801 |
Hammond AH, Fry JR: Toxicity of precocene II in rat hepatocyte cultures: effects of serum and culture time. Toxicol Lett. 1994 Feb 15;70(3):337-42.
Omission of serum from culture medium markedly increased the toxicity of the cytochrome P450-activated toxin precocene II in 24-h cultures of rat hepatocytes. In addition, the depletion of glutathione and inhibition of cytochrome P450 in 24-h cultures increased and decreased, respectively, the toxicity of precocene II, as seen in vivo. Precocene II was also toxic to 72-h cultures, although the effect of the serum-free medium was attenuated. However, depletion of glutathione and inhibition of P450 had little or no effect on the toxicity in 72-h cultures. Therefore, the mechanism of precocene II-induced cell death appears to change with time in culture. |
112(1,2,2,2) |