| 3144975 |
Trower MK, Sariaslani FS, Kitson FG: Xenobiotic oxidation by cytochrome P-450-enriched extracts of Streptomyces griseus. Biochem Biophys Res Commun. 1988 Dec 30;157(3):1417-22.
Crude extracts of Streptomyces griseus grown on soybean flour-enriched medium contain high levels of cytochrome P-450. The cytochrome P-450-enriched fractions, obtained by ammonium sulfate fractionation (30-50% saturation), catalyze the NADPH-dependent oxidation of a variety of xenobiotics when complemented with both spinach ferredoxin:NADP+ oxidoreductase and spinach ferredoxin. Reactions observed are aromatic, benzylic and alicyclic hydroxylations, O-dealkylation, non-aromatic double bond epoxidation, N-oxidation and N-acetylation. |
3(0,0,0,3) |