| 14510482 |
Liu M, Asanuma H, Komiyama M: Effective photo-regulation of transcription reaction by SP6 RNA polymerase with modified DNA tethering multiple azobenzenes. Nucleic Acids Res Suppl. 2003;(3):265-6.
Effective photo-regulation of transcription reaction by SP6 RNA polymerase (RNAP) was achieved with photo-responsive SP6 promoter tethering two azobenzenes. With one azobenzene in either TATA or RNAP binding region of the SP6 promoter, photo-regulation activity was very small. But when two azobenzenes were introduced into both regions, efficient photo-regulation of transcription was attained: transcription proceeded 3.5 fold faster under UV irradiation than under dark. |
83(1,1,1,3) |