| 12903112 |
Asanuma H, Liu M, Tamaru D, Liang X, Komiyama M: Photo-regulation of transcription by RNA polymerase with azobenzene-tethered promoter. Nucleic Acids Res Suppl. 2002;(2):75-6.
Photo-regulation of transcription reaction by T7-RNA polymerase was investigated by introducing an azobenzene residue into the non-template strand of T7-promoter. It was found that transcription was strongly interfered under dark or visible light irradiation, whereas it efficiently proceeded after UV irradiation. With this photo-responsive promoter, on-off switching of transcription by light irradiation was achieved. This strategy was also effective for the photo-regulation of SP6-RNA polymerase. |
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