| 18551504 |
Peccinini RG, Ximenes VF, Cesarino EJ, Lanchote VL: Stereoselective analysis of carvedilol in human plasma and urine using HPLC after chiral derivatization. Biopharm Drug Dispos. 2008 Jul;29(5):280-8.
An enantioselective high-performance liquid chromatographic method for the analysis of carvedilol in plasma and urine was developed and validated using (-)-menthyl chloroformate (MCF) as a derivatizing reagent. Chloroform was used for extraction, and analysis was performed by HPLC on a C18 column with a fluorescence detector. The quantitation limit was 0.25 ng/ml for S (-)-carvedilol in plasma and 0.5 ng/ml for R (+)-carvedilol in plasma and for both enantiomers in urine. The method was applied to the study of enantioselectivity in the pharmacokinetics of carvedilol administered in a multiple dose regimen (25 mg/12 h) to a hypertensive elderly female patient. The data obtained demonstrated highest plasma levels for the R (+)-carvedilol (AUC (SS) (0-12) 75.64 vs 37.29 ng/ml). The enantiomeric ratio R (+)/S (-) was 2.03 for plasma and 1.49 for urine (Ae (0-12) 17.4 vs 11.7 microg). |
6(0,0,1,1) |