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Delevskii IuP, Zinchenko AA: [Comparison of the antigenic spectrum A1, A2, and Ax erythrocytes: inhibition of mab with glucoconjugates of lipid and protein nature with different isoelectric properties]. Klin Lab Diagn. 2007 Dec;(12):48-53.
The inhibition of monoclonal antibodies (MAbs) (anti-A1 2-24, anti-A 2-22, anti- 2-19, and anti- A 2-18; Workshop IV) by glycotopes (glycoconjugates) obtained by the enzymatic treatment of A1, A2, and Ax erythrocytes and by chloroform-methanol method from the membranes of these erythrocytes, followed by ion-exchange gel chromatography was evaluated. According to specificity and isoelectric properties, glycoconjugates (in the sequence of desorption from an anion exchanger) of glycoprotein (A (pr-00), A (pr-0), A (pr-1), A (pr-3)) and glycosphingolipid (A1p,00, A (1p-0), A (1p-1), A (1p-3), A (1p-3)) origin were identified. A1 erythrocytes showed the broad-spectrum inhibiting activity of glycoconjugates, which being at maximum with acid A (1p-00) and A (pr-3) (the ionic points of pH 6.55 and 6.45), as well as with A (lp-00) and A (1p-00) 1 The inhibition of A (pr-3) from A2 was significantly weaker that that from A1. An A (pr-3) fraction from Ax erythrocytes inhibited as strongly as in A1 and A2. The inhibition of A (pr-3p) from Ax either failed to develop at all (MAb 2-19) or turned out be weakened (MAbs 2-22 and 2-18). Thus, the cascade decrease in agglutinogenicity in the order of A1, A2, and Ax correlated with the reduction in the inhibiting capacity of A (pr-3) in all the study groups, including Ax. |
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