Protein Information

Name RGL2
Synonyms HKE1.5; KE1.5; RAB2L; RAS associated protein RAB2L; RGL 2; RGL2; Ral guanine nucleotide dissociation stimulator like 2; RalGDS like factor…

Compound Information

Name paclobutrazol
CAS

Reference List

PubMed Abstract RScore(About this table)
11877383 Lee S, Cheng H, King KE, Wang W, He Y, Hussain A, Lo J, Harberd NP, Peng J: Gibberellin regulates Arabidopsis seed germination via RGL2, a GAI/RGA-like gene whose expression is up-regulated following imbibition. Genes Dev. 2002 Mar 1;16(5):646-58.

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17384169 Ariizumi T, Steber CM: Seed germination of GA-insensitive sleepy1 mutants does not require RGL2 protein disappearance in Arabidopsis. Plant Cell. 2007 Mar;19(3):791-804. Epub 2007 Mar 23.


We explore the roles of gibberellin (GA) signaling genes SLEEPY1 (SLY1) and RGA-LIKE2 (RGL2) in regulation of seed germination in Arabidopsis thaliana, a plant in which the hormone GA is required for seed germination.
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18941053 Piskurewicz U, Jikumaru Y, Kinoshita N, Nambara E, Kamiya Y, Lopez-Molina L: The gibberellic acid signaling repressor RGL2 inhibits Arabidopsis seed germination by stimulating abscisic acid synthesis and ABI5 activity. Plant Cell. 2008 Oct;20(10):2729-45. Epub 2008 Oct 21.

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16339853 Ward JM, Smith AM, Shah PK, Galanti SE, Yi H, Demianski AJ, van der Graaff E, Keller B, Neff MM: A new role for the Arabidopsis AP2 transcription factor, LEAFY PETIOLE, in gibberellin-induced germination is revealed by the misexpression of a homologous gene, SOB2/DRN-LIKE. Plant Cell. 2006 Jan;18(1):29-39. Epub 2005 Dec 9.

These results suggest LEP is a positive regulator of GA-induced germination acting independently of RGL2.
In addition, lep-1 is less responsive to GA and more sensitive to the GA biosynthesis inhibitor paclobutrazol, indicating that LEP is a positive regulator of GA-induced germination.
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18363782 Kim SG, Lee AK, Yoon HK, Park CM: A membrane-bound NAC transcription factor NTL8 regulates gibberellic acid-mediated salt signaling in Arabidopsis seed germination. Plant J. 2008 Jul;55(1):77-88.


Interestingly, NTL8 is significantly induced during cold imbibition, but the induction declines quickly in germinating seeds, like RGL2.
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11826301 Wen CK, Chang C: Arabidopsis RGL1 encodes a negative regulator of gibberellin responses. Plant Cell. 2002 Jan;14(1):87-100.

In Arabidopsis, the DELLA subfamily of GRAS regulatory genes consists of GAI, RGA, RGA-LIKE1 (RGL1), RGL2, and RGL3.
Conversely, a loss-of-function rgl1 line had reduced GA4 expression and exhibited GA-independent activation of seed germination, leaf expansion, flowering, stem elongation, and floral development, as detected by resistance to the GA biosynthesis inhibitor paclobutrazol.
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18162586 Toh S, Imamura A, Watanabe A, Nakabayashi K, Okamoto M, Jikumaru Y, Hanada A, Aso Y, Ishiyama K, Tamura N, Iuchi S, Kobayashi M, Yamaguchi S, Kamiya Y, Nambara E, Kawakami N: High temperature-induced abscisic acid biosynthesis and its role in the inhibition of gibberellin action in Arabidopsis seeds. Plant Physiol. 2008 Mar;146(3):1368-85. Epub 2007 Dec 27.


In addition, the thermoinhibition-resistant germination phenotype of aba2-1 seeds is suppressed by a GA biosynthesis inhibitor, paclobutrazol.
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