Protein Information

Name S100A4
Synonyms 18A2; 42A; CAPL; Calcium placental protein; Calvasculin; MTS 1; MTS1; Malignant transformation suppression 1…

Compound Information

Name cycloheximide
CAS

Reference List

PubMed Abstract RScore(About this table)
19604397 Hugo HJ, Wafai R, Blick T, Thompson EW, Newgreen DF: Staurosporine augments EGF-mediated EMT in PMC42-LA cells through actin depolymerisation, focal contact size reduction and Snail1 induction - a model for cross-modulation. BMC Cancer. 2009 Jul 15;9:235.

Gene expression analysis by MT-PCR indicated that ST repressed many genes which were induced by EGF (EGFR, CAV1, CTGF, CYR61, CD44, S100A4) and induced genes which alter the actin cytoskeleton (NLF1, NLF2, EPHB4).
METHODS: PMC42-LA cells were treated for 72 h with 10 ng/ml EGF, 40 nM ST, or both, and assessed for expression of E-cadherin repressor genes (Snail1, Snail2, Zeb1/deltaEF1) and EMT-related genes by QRT-PCR, multiplex tandem PCR (MT-PCR) and immunofluorescence +/- cycloheximide.
1(0,0,0,1) Details
19248116 Yammani RR, Long D, Loeser RF: Interleukin-7 stimulates secretion of S100A4 by activating the JAK/STAT signaling pathway in human articular chondrocytes. Arthritis Rheum. 2009 Mar;60(3):792-800.

Pretreatment of chondrocytes with a JAK-3 inhibitor or with cycloheximide blocked the IL-7-mediated secretion of S100A4, but pretreatment with brefeldin A did not.
38(0,1,1,8) Details