| Name | protein kinase C (protein family or complex) |
|---|---|
| Synonyms | Protein kinase C; PKC |
| Name | cycloheximide |
|---|---|
| CAS |
| PubMed | Abstract | RScore(About this table) | |
|---|---|---|---|
| 18187554 | Yang D, Guo J, Divieti P, Shioda T, Bringhurst FR: CBP/p300-interacting protein CITED1 modulates parathyroid hormone regulation of osteoblastic differentiation. Endocrinology. 2008 Apr;149(4):1728-35. Epub 2008 Jan 10. Stimulation of CITED1 mRNA by PTH was transient, peaking at 4 h, concentration dependent, and blocked by actinomycin D but not cycloheximide. The stimulation was mimicked by forskolin, phorbol ester, and the cAMP-selective PTH analog [G (1),R (19)] hPTH (1-28) and inhibited completely by the protein kinase A inhibitor, H89 and partially by phorbol ester-induced protein kinase C depletion. |
1(0,0,0,1) | Details |
| 17064352 | Bobrovskaya L, Gilligan C, Bolster EK, Flaherty JJ, Dickson PW, Dunkley PR: Sustained phosphorylation of tyrosine hydroxylase at serine 40: a novel mechanism for maintenance of catecholamine synthesis. J Neurochem. 2007 Jan;100(2):479-89. Epub 2006 Oct 25. The protein kinase (s) responsible for TH phosphorylation at Ser40 switched from being protein kinase C (PKC) independent in the acute phase to PKC dependent in the sustained phase. |
1(0,0,0,1) | Details |
| 17178924 | Moreau ME, Bawolak MT, Morissette G, Adam A, Marceau F: Role of nuclear factor-kappaB and protein kinase C signaling in the expression of the kinin B1 receptor in human vascular smooth muscle cells. Mol Pharmacol. 2007 Mar;71(3):949-56. Epub 2006 Dec 18. Various stimulatory compounds that increase receptor mRNA stability only (human and bovine sera, cycloheximide) or that stimulate NF-kappaB nuclear translocation and both mRNA concentration and stability [interleukin (IL)-1beta, phorbol 12- 13- (PMA)] all increased the density of binding sites for the tritiated B1 receptor agonist [3H] -des-Arg9-bradykinin (without change in receptor affinity) in cell-based assays. |
4(0,0,0,4) | Details |
| 15770661 | Wang HS, Tung WH, Tang KT, Wong YK, Huang GJ, Wu JC, Guo YJ, Chen CC: TGF-beta induced synthesis in orbital fibroblasts involves protein kinase C betaII activation in vitro. J Cell Biochem. 2005 May 15;95(2):256-67. Stimulation by TGF-beta and PMA were dependent on protein synthesis and their effects were inhibited by cycloheximide. |
1(0,0,0,1) | Details |
| 20339915 | Ji L, Chauhan A, Chauhan V: Induces Expression of Cytoplasmic Gelsolin in SH-SY5Y and HEK-293 Cells. Neurochem Res. 2010 Mar 26. We report here that ionophore A23187 induces the expression of cytoplasmic gelsolin (c-gelsolin), and that protein kinase C (PKC) is involved in the up-regulation of c-gelsolin. -mediated up-regulation of c-gelsolin was inhibited by cycloheximide (a general inhibitor of protein synthesis). |
1(0,0,0,1) | Details |
| 17143556 | Lai YL, Yamaguchi M: Phytocomponent inhibits osteoclast-like cell formation in mouse bone marrow cultures. Int J Mol Med. 2007 Jan;19(1):123-8. The inhibitory effect of HCA (10 (-6) or 10 (-5) M) on RANKL plus M-CSF-induced osteoclast-like cell formation was not observed in the presence of cycloheximide (10 (-7) M), an inhibitor of protein synthesis in the transcriptional process, or 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (10 (-6) M), an inhibitor of transcription. HCA (10 (-6) or 10 (-5) M) significantly inhibited osteoclast-like cell formation induced by dibutyryl cyclic adenosine monophosphate (10 (-5) M) or phorbol 12- 13- (10 (-6) M), an activator of protein kinase C. |
1(0,0,0,1) | Details |
| 16888033 | Garcia CS, Curiel RE, Mwatibo JM, Pestka S, Li H, Espinoza-Delgado I: The antineoplastic agent bryostatin-1 differentially regulates IFN-gamma receptor subunits in monocytic cells: transcriptional and posttranscriptional control of IFN-gamma R2. J Immunol. 2006 Aug 15;177(4):2707-16. Bryostatin-1 (Bryo-1) is a potent ligand and modulator of protein kinase C that exerts antineoplastic and immunomodulatory activities both in vitro and in vivo. Bryo-1-induced up-regulation of IFN-gammaR2 mRNA levels is not dependent on de novo protein synthesis as shown by cell treatment with the protein-synthesis inhibitor cycloheximide. |
1(0,0,0,1) | Details |
| 19376214 | Ridley W, Matsuoka M: -induced cyclooxygenase-2 expression and production in A549 human pulmonary epithelial cells. Toxicol Lett. 2009 Aug 10;188(3):180-5. Epub 2009 Apr 17. NaF-induced accumulation of COX-2 transcript was abolished by actinomycin D, but not cycloheximide. Phosphorylated forms of mitogen-activated protein kinases (MAPKs)-including extracellular signal-regulated protein kinase (ERK), c-Jun NH (2)-terminal kinase, and p38-increased after NaF exposure, while treatment with the MAPK/ERK kinase inhibitor U0126 and the p38 inhibitor SB203580 markedly suppressed COX-2 expression. |
1(0,0,0,1) | Details |
| 15944291 | Wojciechowski W, Li H, Marshall S, Dell'Agnola C, Espinoza-Delgado I: Enhanced expression of CD20 in human tumor B cells is controlled through ERK-dependent mechanisms. J Immunol. 2005 Jun 15;174(12):7859-68. The effect of bryostatin-1 on CD20 expression in non-Hodgkin's lymphoma cells was mediated through the MAPK kinase/ERK signal transduction pathway and involved protein kinase C, but was independent of p38 MAPK and was insensitive to dexamethasone. |
1(0,0,0,1) | Details |
| 17266789 | Toranzo GS, Bonilla F, Zelarayan L, Oterino J, Buhler MI: Activation of maturation promoting factor in Bufo arenarum oocytes: injection of mature cytoplasm and germinal vesicle contents. Zygote. 2006 Nov;14(4):305-16. The injection of cytoplasm containing active MPF is sufficient to activate an amplification loop that requires the activation of cdc25 and protein kinase C, the decrease in cAMP levels, and is independent of protein synthesis. |
1(0,0,0,1) | Details |
| 15666812 | Clark BJ, Li J: Janus kinase 2 signaling in the angiotensin II-dependent activation of StAR expression. Endocr Res. 2004 Nov;30(4):685-93. Using StAR promoter-reporter gene activity and pharmacological inhibition of signaling pathways, we have shown that Ang II-stimulated StAR transcription in H295R cells is dependent upon both influx of external Ca2+ and kinase signaling and is enhanced by protein kinase C and mitogen-activated protein kinase (ERK1/2) activation. The Ang II-dependent, but not (Bu) 2cAMP-dependent, induction of StAR mRNA was also blocked by AG490 and shown to be sensitive to cycloheximide treatment. |
1(0,0,0,1) | Details |
| 17329956 | Matsumoto E, Hatanaka M, Bohgaki M, Maeda S: PKC pathway and ERK/MAPK pathway are required for induction of cyclin D1 and p21Waf1 during 12-o-tetradecanoylphorbol 13--induced differentiation of myeloleukemia cells. Kobe J Med Sci. 2006;52(6):181-94. Induction of cyclin D1 expression in TPA-treated HL60 cells was inhibited with protein kinase C (PKC) inhibitor bisindolylmaleimide I and mitogen activated protein kinase kinase (MEK) inhibitor PD98059. |
1(0,0,0,1) | Details |
| 16571380 | Huwiler A, Doll F, Ren S, Klawitter S, Greening A, Romer I, Bubnova S, Reinsberg L, Pfeilschifter J: increases sphingosine kinase-1 expression and activity in the human arterial endothelial cell line EA.hy 926 by a PKC-alpha-dependent mechanism. Biochim Biophys Acta. 2006 Mar;1761(3):367-76. Epub 2006 Mar 10. A similar upregulation of SK-1 is also observed with the direct protein kinase C activator 12-O-tetradecanoylphorbol-13-acetate (TPA). The increased SK-1 protein expression is due to stimulated de novo synthesis since cycloheximide inhibited the delayed SK-1 protein upregulation. |
1(0,0,0,1) | Details |
| 15654655 | Sabbieti MG, Marchetti L, Gabrielli MG, Menghi M, Materazzi S, Menghi G, Raisz LG, Hurley MM: Prostaglandins differently regulate FGF-2 and FGF receptor expression and induce nuclear translocation in osteoblasts via MAPK kinase. Cell Tissue Res. 2005 Feb;319(2):267-78. Epub 2004 Dec 10. Pre-treatment with cycloheximide blocks nuclear labelling for FGF-2 in response to PGF (2alpha). Similar results are obtained by pre-treatment with the protein kinase C (PKC) inhibitor H-7. |
1(0,0,0,1) | Details |
| 15946695 | Han HJ, Heo JS, Lee YJ: ANG II increases 2-deoxyglucose uptake in mouse embryonic stem cells. . Life Sci. 2005 Aug 26;77(15):1916-33. Actinomycin D and cycloheximide completely blocked the effect of ANG II on 2-DG uptake. Protein kinase C (PKC) inhibitors, staurosporine, bisindolylmaleimide I, and H-7 also blocked ANG II-induced stimulation of 2-DG uptake. |
1(0,0,0,1) | Details |
| 16433893 | Cao YX, Xu CB, Luo GG, Edvinsson L: Up-regulation of alpha1A-adrenoceptors in rat mesenteric artery involves intracellular signal pathways. Basic Clin Pharmacol Toxicol. 2006 Jan;98(1):61-7. Transcriptional inhibitor actinomycin D, translational inhibitor cycloheximide, protein kinase C inhibitors (staurosporine and RO31-8220) and mitogen-activated protein kinase (MAPK) pathway inhibitors (SB386023, U0126 and SB239063) prevented the increase in NA-induced contractions. |
32(0,1,1,2) | Details |
| 16297161 | Hormdee D, Nagasawa T, Kiji M, Yashiro R, Kobayashi H, Koshy G, Noguchi K, Nitta H, Ishikawa I: Protein kinase-A-dependent osteoprotegerin production on interleukin-1 stimulation in human gingival fibroblasts is distinct from periodontal ligament fibroblasts. Clin Exp Immunol. 2005 Dec;142(3):490-7. Human gingival fibroblasts (HGF) and periodontal ligament fibroblasts (PDL) were stimulated with IL-1alpha with or without protein synthesis inhibitor cycloheximide (CHX), protein kinase A (PKA) inhibitors, protein kinase C (PKC) inhibitors and (2) (PGE (2)) inhibitor. |
31(0,1,1,1) | Details |
| 18948121 | Lim SK, Park MJ, Jung HK, Park AY, Kim DI, Kim JC, Bae CS, Kim KY, Yoon KC, Han HJ, Park SH: Bradykinin stimulates uptake via both B1R and B2R activation in a human retinal pigment epithelial cells. Life Sci. 2008 Dec 5;83(23-24):761-70. Epub 2008 Sep 30. Furthermore, the BK-induced increase in COX-2 expression was blocked by the PI-3 kinase inhibitors wortmannin and LY294002, Akt inhibitor, and the protein kinase C (PKC) inhibitors staurosporine and bisindolylmaleimide I, suggesting the role of PI-3 kinase and PKC in this process. |
1(0,0,0,1) | Details |
| 17331500 | Bian ZM, Elner SG, Elner VM: Regulation of VEGF mRNA expression and protein secretion by TGF-beta2 in human retinal pigment epithelial cells. Exp Eye Res. 2007 May;84(5):812-22. Epub 2007 Jan 9. Induced VEGF expression was completely abrogated by inhibitors of protein kinase C (PKC) (Ro318220), nuclear factor-kappaB (NF-kappaB) phenethyl ester (CAPE)], and reactive species (ROS) [N-acetyl- (Nac) and diphenyleneiodonium (DPI)]. Stimulation of VEGF expression by TGF-beta2 was blocked by cycloheximide, suggesting that de novo protein synthesis is required. |
1(0,0,0,1) | Details |
| 19843871 | Qi D, Cai K, Wang O, Li Z, Chen J, Deng B, Qian L, Le Y: Fatty acids induce amylin expression and secretion by pancreatic {beta}-cells. Am J Physiol Endocrinol Metab. 2009 Oct 20. Pretreatment of MIN6 cells with Ca (2+) chelator (EGTA, BAPTA/AM), protein kinase C (PKC) inhibitor Go6976, or protein synthesis inhibitor cycloheximide significantly inhibited FA-induced amylin mRNA expression. |
31(0,1,1,1) | Details |
| 15695798 | Paik JY, Lee KH, Ko BH, Choe YS, Choi Y, Kim BT: stimulates 18F-FDG uptake in human endothelial cells through increased hexokinase activity and GLUT1 expression. J Nucl Med. 2005 Feb;46(2):365-70. SNP-stimulated (18) F-FDG uptake was abolished by cotreatment with cycloheximide, the kinase inhibitor the phosphatidylinositol-3 kinase (PI3K) inhibitor wortmannin, or the protein kinase C inhibitor staurosporine. |
31(0,1,1,1) | Details |
| 17885094 | Canas N, Valero T, Villarroya M, Montell E, Verges J, Garcia AG, Lopez MG: protects SH-SY5Y cells from oxidative stress by inducing heme oxygenase-1 via phosphatidylinositol 3-kinase/Akt. J Pharmacol Exp Ther. 2007 Dec;323(3):946-53. Epub 2007 Sep 20. Taken together, these results show that CS can protect SH-SY5Y cells under oxidative stress conditions by activating protein kinase C, which phosphorylates Akt that, via the phosphatidylinositol 3-kinase/Akt pathway, induces the synthesis of the antioxidant protein heme oxygenase-1. The protective effects of CS were prevented by chelerythrine, 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one (LY294002), cycloheximide, and Sn (IV)- |
1(0,0,0,1) | Details |
| 16543731 | Heo JS, Han HJ: PKC and MAPKs pathways mediate EGF-induced stimulation of 2-deoxyglucose uptake in mouse embryonic stem cells. Cell Physiol Biochem. 2006;17(3-4):145-58. Epub 2006 Mar 14. Actinomycin D and cycloheximide completely blocked the effect of EGF on 2-DG uptake. In addition, EGF effect was blocked by neomycin and U 73122 [phospholipase C (PLC) inhibitors] as well as staurosporine and bisindolylmaleimide I [protein kinase C (PKC) inhibitors]. |
1(0,0,0,1) | Details |
| 17065529 | Tezel TH, Geng L, Kaplan HJ, Del Priore LV: Retinal pigment epithelium rescues vascular endothelium from -induced apoptosis. Invest Ophthalmol Vis Sci. 2006 Nov;47(11):5075-87. Apoptosis rates were determined at 24 hours, and the effect of inhibition of protein synthesis and activation of protein kinase C on apoptosis was investigated by supplying culture medium with 0.1 mg/mL cycloheximide and 10 nM phorbol |
31(0,1,1,1) | Details |
| 18479958 | Lee MY, Han HJ: Galectin-1 upregulates glucose transporter-1 expression level via protein kinase C, phosphoinositol-3 kinase, and mammalian target of rapamycin pathways in mouse embryonic stem cells. Int J Biochem Cell Biol. 2008;40(11):2421-30. Epub 2008 Apr 6. Moreover, galectin-1 increased glucose transporter-1 mRNA and protein expression levels, which were inhibited by a disruption in transcription by actinomycin D and translation by the cycloheximide. |
4(0,0,0,4) | Details |
| 16895520 | Carmosino M, Procino G, Tamma G, Mannucci R, Svelto M, Valenti G: Trafficking and phosphorylation dynamics of AQP4 in -treated human gastric cells. Biol Cell. 2007 Jan;99(1):25-36. Using an endo-exocytosis assay we showed that, after washed out, internalized AQP4 recycled back to the cell surface, even in cells in which de novo protein synthesis was inhibited by cycloheximide. |
0(0,0,0,0) | Details |
| 16464174 | Malakooti J, Sandoval R, Amin MR, Clark J, Dudeja PK, Ramaswamy K: Transcriptional stimulation of the human NHE3 promoter activity by PMA: PKC independence and involvement of the transcription factor EGR-1. Biochem J. 2006 Jun 1;396(2):327-36. Pretreatment with actinomycin D or cycloheximide blocked the up-regulation of the NHE3 mRNA by PMA, indicating that the increased level of NHE3 mRNA expression is regulated by transcriptional activation and is dependent on de novo protein synthesis. 5'-Deletion of the promoter region and transfection analysis in C2BBe1 cells revealed that the PMA effect is mediated through a GC-rich DNA region between nt -88 and -69. |
0(0,0,0,0) | Details |
| 15569273 | Luo G, Xu CB, Cao YX, Edvinsson L: Transcriptional up-regulation in expression of 5-hydroxytryptamine2A and transcriptional down-regulation of angiotensin II type 1 receptors during organ culture of rat mesenteric artery. Basic Clin Pharmacol Toxicol. 2004 Dec;95(6):280-7. The purpose of this study was to investigate in rat mesenteric artery if there is up-regulation of receptors and angiotensin II receptors and the potential role of protein kinase C activation in the smooth muscle cells during organ culture. |
2(0,0,0,2) | Details |
| 17156130 | Kalfon L, Youdim MB, Mandel SA: Green tea polyphenol (-) - promotes the rapid protein kinase C- and proteasome-mediated degradation of Bad: implications for neuroprotection. J Neurochem. 2007 Feb;100(4):992-1002. Epub 2006 Nov 29. Co-treatment with and the protein synthesis inhibitor cycloheximide prominently shortened Bad half-life, with as little as 30% of the Bad protein content remaining after 2 h, suggesting an effect of on Bad protein degradation. |
2(0,0,0,2) | Details |
| 17596302 | Ye J, Gradoville L, Daigle D, Miller G: De novo protein synthesis is required for lytic cycle reactivation of Epstein-Barr virus, but not Kaposi's sarcoma-associated herpesvirus, in response to histone deacetylase inhibitors and protein kinase C agonists. J Virol. 2007 Sep;81(17):9279-91. Epub 2007 Jun 27. Using Northern blotting and quantitative reverse transcriptase PCR, we measured the kinetics of expression of the lytic cycle activator genes and determined whether abundance of mRNAs encoding these genes from either virus was reduced by treatment with cycloheximide (CHX), an inhibitor of protein synthesis. |
0(0,0,0,0) | Details |
| 19716839 | Charlson AT, Zeliadt NA, Wattenberg EV: Extracellular signal regulated kinase 5 mediates signals triggered by the novel tumor promoter palytoxin. Toxicol Appl Pharmacol. 2009 Dec 1;241(2):143-53. Epub 2009 Aug 28. Cycloheximide, okadaic acid, and orthovanadate did not mimic the effect of palytoxin on ERK5. |
0(0,0,0,0) | Details |
| 15936242 | Lee YS, Paek KS, Kang ES, Jang HS, Kim HJ, Kang YJ, Kim JH, Lee HT, Lee JH, Chang KC, Nishinaka T, Seo HG: Involvement of nuclear factor kappaB in up-regulation of aldose reductase gene expression by 12-O-tetradecanoylphorbol-13- in HeLa cells. Int J Biochem Cell Biol. 2005 Nov;37(11):2297-309. Inhibitors of protein kinase C, bisindolylmaleimide I and calphostin C, as well as inhibitors of kinase, and tyrphostin A23, significantly attenuated 12-O-tetradecanoylphorbol-13--induced increase in aldose reductase mRNA. |
2(0,0,0,2) | Details |
| 19920324 | Ji L, Chauhan A, Chauhan V: Upregulation of Cytoplasmic Gelsolin, an Amyloid-beta-Binding Protein, Under Oxidative Stress Conditions: Involvement of Protein Kinase C. J Alzheimers Dis. 2009 Nov 17. Pretreatment of cells with cycloheximide (an inhibitor of protein synthesis) resulted in significant inhibition of H |
2(0,0,0,2) | Details |
| 20157239 | Ji L, Chauhan A, Chauhan V: Upregulation of cytoplasmic gelsolin, an amyloid-beta-binding protein, under oxidative stress conditions: involvement of protein kinase C. J Alzheimers Dis. 2010 Jan;19(3):829-38. Pretreatment of cells with cycloheximide (an inhibitor of protein synthesis) resulted in significant inhibition of H (2) O (2) induced c-gelsolin expression, suggesting the possible de novo synthesis of c-gelsolin in cells. |
2(0,0,0,2) | Details |
| 19179479 | Armstrong SP, Caunt CJ, McArdle CA: Gonadotropin-releasing hormone and protein kinase C signaling to ERK: spatiotemporal regulation of ERK by docking domains and dual-specificity phosphatases. Mol Endocrinol. 2009 Apr;23(4):510-9. Epub 2009 Jan 29. Cycloheximide caused more sustained effects of GnRH and phorbol ester on ppERK, suggesting termination by nuclear-inducible DUSPs. |
2(0,0,0,2) | Details |
| 16926447 | Lanaspa MA, Giral H, Breusegem SY, Halaihel N, Baile G, Catalan J, Carrodeguas JA, Barry NP, Levi M, Sorribas V: Interaction of MAP17 with NHERF3/4 induces translocation of the renal Na/Pi IIa transporter to the trans-Golgi. Am J Physiol Renal Physiol. 2007 Jan;292(1):F230-42. Epub 2006 Aug 22. Inhibition of protein kinase C (PKC) prevented expression of the three proteins in the TGN. |
1(0,0,0,1) | Details |
| 19693770 | Tsai JH, Hwang JM, Ying TH, Shyu JC, Tsai CC, Hsieh YS, Wang YW, Liu JY, Kao SH: The activation of matrix metalloproteinase-2 induced by protein kinase C alpha in decidualization. J Cell Biochem. 2009 Oct 15;108(3):547-54. This study investigated the protein kinase C (PKC) and matrix metalloproteinase-2 (MMP-2) in the development of deciduomata in pseudo-pregnant and pregnant rats. Moreover, this response was inhibited by the PKC inhibitor H7, the PKC alpha specific inhibitor Go-6976 and the translation inhibitor cycloheximide, but not by the transcription inhibitor actinomycin D or the replication inhibitor mitomycin C. |
1(0,0,0,1) | Details |
| 18404734 | Tattersall AL, Wilkins RJ: Modulation of Na+-H+ exchange isoforms NHE1 and NHE3 by insulin-like growth factor-1 in isolated bovine articular chondrocytes. J Orthop Res. 2008 Nov;26(11):1428-33. NHE3 activity was not detected in IGF-1-treated cells that had been incubated with the protein synthesis inhibitor cycloheximide, although NHE1 activity was unaffected. For raised hydrostatic pressure, a decrease in NHE1 activity was responsible, and was prevented by the protein kinase C inhibitor chelerythrine. |
1(0,0,0,1) | Details |
| 16075364 | Phelps E, Bezouglaia O, Tetradis S, Nervina JM: Parathyroid hormone induces receptor activity modifying protein-3 (RAMP3) expression primarily via 3',5'-cyclic adenosine monophosphate signaling in osteoblasts. Calcif Tissue Int. 2005 Aug;77(2):96-103. Epub 2005 Aug 11. PTH induces osteoblastic gene expression through PTHR1, a heptahelical receptor that triggers cyclic (cAMP)-protein kinase A (PKA), protein kinase C (PKC), and signaling. Protein synthesis inhibition with 3 microg/mL cycloheximide did not affect PTH-induced RAMP3 mRNA levels. |
1(0,0,0,1) | Details |
| 16805793 | Sugano T, Yanagita T, Yokoo H, Satoh S, Kobayashi H, Wada A: Enhancement of insulin-induced PI3K/Akt/GSK-3beta and ERK signaling by neuronal nicotinic receptor/PKC-alpha/ERK pathway: up-regulation of IRS-1/-2 mRNA and protein in chromaffin cells. J Neurochem. 2006 Jul;98(1):20-33. The increase in IRS-1/IRS-2 levels induced by was prevented by nicotinic acetylcholine receptor (nAChR) antagonists, the Ca (2+) chelator 1,2-bis (2-aminophenoxy)-ethane-N,N,N',N'-tetra- tetrakis-acetoxymethyl ester, cycloheximide or actinomycin D. increased IRS-1 and IRS-2 mRNA levels by approximately 57 and approximately 50%, and this was prevented by conventional protein kinase C (cPKC) inhibitor Go6976, or ERK kinase inhibitors PD98059 and U0126. |
1(0,0,0,1) | Details |
| 15561096 | Rivedal E, Leithe E: Connexin43 synthesis, phosphorylation, and degradation in regulation of transient inhibition of gap junction intercellular communication by the phorbol ester TPA in rat liver epithelial cells. Exp Cell Res. 2005 Jan 15;302(2):143-52. The initial block in GJIC has been attributed to protein kinase C (PKC) mediated phosphorylation of connexin gap junction proteins, including connexin43 (Cx43). We show that coincubation of TPA with the protein synthesis inhibitor cycloheximide or the transcription inhibitor actinomycin D results in synergistic enhancement of the level of activated ERK1/2. |
1(0,0,0,1) | Details |
| 15928807 | Kang ES, Kim HJ, Paek KS, Jang HS, Chang KC, Lee JH, Nishinaka T, Yabe-Nishimura C, Seo HG: Phorbol ester up-regulates aldose reductase expression in A549 cells: a potential role for aldose reductase in cell cycle modulation. Cell Mol Life Sci. 2005 May;62(10):1146-55. The TPA-induced increase in mRNA level and promoter activity of the AR gene was significantly attenuated in the presence of an inhibitor of protein kinase C, kinase, or nuclear factor kappaB (NF-kappaB). |
1(0,0,0,1) | Details |
| 19070612 | Cazarolli LH, Folador P, Moresco HH, Brighente IM, Pizzolatti MG, Silva FR: Mechanism of action of the stimulatory effect of -6-C-(2''-O-alpha-l-rhamnopyranosyl)-beta-L-fucopyranoside on 14C- uptake. Chem Biol Interact. 2009 May 15;179(2-3):407-12. Epub 2008 Nov 25. The effect of compound 1 on uptake was completely nullified by wortmannin, an inhibitor of phosphatidylinositol 3-kinase (PI3K), RO318220, an inhibitor of protein kinase C (PKC), PD98059, a specific inhibitor of mitogen-activated protein kinase (MEK), cycloheximide, an inhibitor of protein synthesis, and colchicine, a microtubule-depolymerizing agent. The effect of compound 1 on uptake was completely nullified by wortmannin, an inhibitor of phosphatidylinositol 3-kinase (PI3K), RO318220, an inhibitor of protein kinase C (PKC), PD98059, a specific inhibitor of mitogen-activated protein kinase (MEK), cycloheximide, an inhibitor of protein synthesis, and colchicine, a microtubule-depolymerizing agent. |
1(0,0,0,1) | Details |
| 17438304 | Sonalker PA, Jackson EK: via beta-adrenoceptors, regulates bumetanide-sensitive cotransporter type 1 expression in thick ascending limb cells. Hypertension. 2007 Jun;49(6):1351-7. Epub 2007 Apr 16. Treatment with either alone or in the presence of actinomycin D or cycloheximide, had no effect on cotransporter mRNA levels. To further elucidate the mechanism, thick ascending limb cells were treated with in the presence of phentolamine (alpha-adrenoceptor blocker), (beta-adrenoceptor blocker), SQ22536 (adenylyl cyclase inhibitor), PD098059 (mitogen-activated protein kinase pathway inhibitor), H-89 (protein kinase A inhibitor), or staurosporine (protein kinase C inhibitor). |
1(0,0,0,1) | Details |
| 16330215 | Fujimoto S, Katsuki H, Kume T, Akaike A: Thrombin-induced delayed injury involves multiple and distinct signaling pathways in the cerebral cortex and the striatum in organotypic slice cultures. Neurobiol Dis. 2006 Apr;22(1):130-42. Epub 2005 Dec 5. These effects were prevented by cycloheximide and actinomycin D but not by a caspase-3 inhibitor. In addition, inhibition of extracelluar signal-regulated kinase (ERK), Src kinase and protein kinase C prevented both neuronal injury in the cortex and shrinkage of the striatum, whereas inhibition of p38 mitogen-activated protein kinase and c-Jun N-terminal kinase prevented shrinkage of the striatum only. |
1(0,0,0,1) | Details |
| 16043662 | Rude MK, Duhaney TA, Kuster GM, Judge S, Heo J, Colucci WS, Siwik DA, Sam F: stimulates matrix metalloproteinases and reactive species in adult rat ventricular cardiomyocytes. Hypertension. 2005 Sep;46(3):555-61. Epub 2005 Jul 25. Protein kinase C (PKC) is implicated in the nongenomic effects of |
1(0,0,0,1) | Details |
| 18719002 | Pritchard DM, Berry D, Przemeck SM, Campbell F, Edwards SW, Varro A: Gastrin increases mcl-1 expression in type I gastric carcinoid tumors and a gastric epithelial cell line that expresses the CCK-2 receptor. Am J Physiol Gastrointest Liver Physiol. 2008 Oct;295(4):G798-805. Epub 2008 Aug 21. Gastrin therefore signals via the CCK-2 receptor, protein kinase C, and MAP kinase to induce expression of antiapoptotic mcl-1 in AGS-G (R) cells, and mcl-1 expression is also increased in human hypergastrinemia-associated type I gastric carcinoid tumors. Gastrin-induced mcl-1 expression was inhibited by the transcription inhibitor actinomycin D and by the protein synthesis inhibitor cycloheximide. |
1(0,0,0,1) | Details |
| 19223651 | Dominguez B, Felix R, Monjaraz E: Upregulation of voltage-gated Na+ channels by long-term activation of the ghrelin-growth hormone secretagogue receptor in clonal GC somatotropes. Am J Physiol Endocrinol Metab. 2009 May;296(5):E1148-56. Epub 2009 Feb 17. Conversely, treatment with a cAMP analog or a protein kinase C activator enhanced both basal and GHS-induced secretion of GH measured by enzyme-linked immunoassay, suggesting that GHRP-6 acting through the ghrelin receptor and different signaling pathways enhances Na (+) channel membrane expression, which favors hormone release from GC somatotropes. |
1(0,0,0,1) | Details |
| 18094537 | Ikewaki N, Tamauchi H, Inoko H: Decrease in CD93 (C1qRp) expression in a human monocyte-like cell line (U937) treated with various apoptosis-inducing chemical substances. Microbiol Immunol. 2007;51(12):1189-200. We previously reported that a protein kinase C activator (PKC), phorbol (PMA), effectively up-regulated CD93 expression on several cultured cell lines and that its regulation was mainly controlled by a PKC delta-isoenzyme. |
1(0,0,0,1) | Details |
| 18651560 | Yuan G, Nanduri J, Khan S, Semenza GL, Prabhakar NR: Induction of HIF-1alpha expression by intermittent hypoxia: involvement of oxidase, Ca2+ signaling, prolyl hydroxylases, and mTOR. J Cell Physiol. 2008 Dec;217(3):674-85. Rapamycin or cycloheximide, blocked increased HIF-1alpha levels during re-oxygenation indicating that mTOR-dependent protein synthesis is required for the persistent elevation of HIF-1alpha levels during re-oxygenation. We further demonstrate that ROS-dependent Ca (2+) signaling pathways involving phospholipase Cgamma (PLCgamma) and protein kinase C activation are required for IH-evoked HIF-1alpha accumulation. |
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| 20042606 | Cardoso LE, Little PJ, Ballinger ML, Chan CK, Braun KR, Potter-Perigo S, Bornfeldt KE, Kinsella MG, Wight TN: Platelet-derived growth factor differentially regulates the expression and post-translational modification of versican by arterial smooth muscle cells through distinct protein kinase C and extracellular signal-regulated kinase pathways. J Biol Chem. 2010 Mar 5;285(10):6987-95. Epub 2009 Dec 30. Interestingly, blocking protein synthesis in the presence of cycloheximide abolished the PDGF effect, but not in the presence of xyloside, indicating that GAG synthesis that results from PKC activation is independent from de novo protein synthesis. |
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| 20207835 | Gonzalez-Montelongo MC, Marin R, Gomez T, Marrero-Alonso J, Diaz M: Androgens Induce Nongenomic Stimulation of Colonic Contractile Activity through Induction of Sensitization and Phosphorylation of LC20 and CPI-17. Mol Endocrinol. 2010 Mar 5. The results using flutamide, finasteride, cycloheximide, and actinomycin D indicate that androgen-induced potentiation is dependent on androgen receptors, requires reduction of to DHT, and occurs independently of transcriptional and translational events. Paralleling these findings, inhibition of Rho-associated Rho kinase (ROK) and/or protein kinase C (PKC) with, respectively, Y27632 and chelerythrine, prevented LC20 phosphorylation and abolished sensitization. |
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| 15547951 | Chiou GY, Fong JC: Synergistic effect of and cyclic AMP on transport in 3T3-L1 adipocytes. J Cell Biochem. 2005 Feb 15;94(3):627-34. The synergistic effect of these two agents was suppressed in the presence of cycloheximide and actinomycin D. The synergistic action of PGF2alpha with 8-bromo cAMP to enhance transport was inhibited by GF109203X, a selective protein kinase C (PKC) inhibitor. |
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| 18303026 | Tatematsu K, Yoshimoto N, Okajima T, Tanizawa K, Kuroda S: Identification of ubiquitin ligase activity of RBCK1 and its inhibition by splice variant RBCK2 and protein kinase Cbeta. J Biol Chem. 2008 Apr 25;283(17):11575-85. Epub 2008 Feb 25. We previously identified a RING-IBR protein, RBCK1, as a protein kinase C (PKC) beta- and zeta-interacting protein, and its splice variant, RBCK2, lacking the C-terminal half including the RING-IBR domain. |
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| 15519494 | Yoshida M, Niwa M, Ishisaki A, Hirade K, Ito H, Shimizu K, Kato K, Kozawa O: Methotrexate enhances -stimulated heat shock protein 27 induction in osteoblasts. Prostaglandins Leukot Essent Fatty Acids. 2004 Dec;71(6):351-62. We previously showed that stimulates the induction of heat shock protein 27 (HSP27) via protein kinase C (PKC)-dependent p38 mitogen-activated protein (MAP) kinase and p44/p42 MAP kinase in osteoblast-like MC3T3-E1 cells. |
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| 16293985 | Sarker KP, Biswas KK, Yamaji K, Yamakuchi M, Hashiguchi T, Lee KY, Maruyama I: Inhibition of thrombin-induced vascular endothelial growth factor production in human neuroblastoma (NB-1) cells by argatroban. Pathophysiol Haemost Thromb. 2005;34(1):41-7. In these cells, we further show that thrombin-induced VEGF secretion was blocked by cycloheximide and actinomycin D, indicating that de novo protein synthesis is essential for this process. Reduced thrombin-induced VEGF secretion upon treatment with LY294002, calphostin C, or BAPTA, further suggests that the process is dependent on phosphatidyl-inositol-3-kinase, protein kinase C, and |
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| 15837527 | Tenhunen O, Szokodi I, Ruskoaho H: Posttranscriptional activation of BNP gene expression in response to increased left ventricular wall stress: role of calcineurin and PKC. Regul Pept. 2005 Jun 30;128(3):187-96. In contrast, the protein synthesis inhibitor cycloheximide increased baseline BNP mRNA levels and abolished the load-induced activation of gene expression. Furthermore, we studied the effects of protein kinase C (PKC), calcineurin and protein phosphatase 2A (PP2A) inhibition to characterize the role of intracellular pathways in the stretch-induced gene expression in the left ventricle. |
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