Protein Information

Name phosphoenolpyruvate carboxylase
Synonyms Full length cDNA clone CS0DC022YA24 of Neuroblastoma of Homo sapiens; PEP carboxykinase; Phosphoenolpyruvate carboxylase; Mitochondrial phosphoenolpyruvate carboxykinase 2; PCK 2; PCK2; PCK2 protein; PEPCK…

Compound Information

Name cycloheximide
CAS

Reference List

PubMed Abstract RScore(About this table)
15604115 Yeagley D, Quinn PG: 3',5'-cyclic adenosine monophosphate response element-binding protein and CCAAT enhancer-binding protein are dispensable for insulin inhibition of phosphoenolpyruvate carboxykinase transcription and for its synergistic induction by protein kinase A and glucocorticoids. Mol Endocrinol. 2005 Apr;19(4):913-24. Epub 2004 Dec 16.

Phosphoenolpyruvate carboxykinase (PEPCK) transcription is induced by cAMP/protein kinase A (PKA) and glucocorticoids [dexamethasone (Dex)] and is inhibited by insulin to regulate blood glucose.
Cycloheximide did not block insulin inhibition, indicating that alternate translation of C/EBPbeta is not required.
3(0,0,0,3) Details
17337522 Bailey KJ, Gray JE, Walker RP, Leegood RC: Coordinate regulation of phosphoenolpyruvate carboxylase and phosphoenolpyruvate carboxykinase by light and CO2 during C4 photosynthesis. Plant Physiol. 2007 May;144(1):479-86. Epub 2007 Mar 2.

The protein synthesis inhibitor, cycloheximide, blocked dark phosphorylation of PEPCK.
87(1,1,1,7) Details
18753284 Feria AB, Alvarez R, Cochereau L, Vidal J, Garcia-Maurino S, Echevarria C: Regulation of phosphoenolpyruvate carboxylase phosphorylation by metabolites and abscisic acid during the development and germination of barley seeds. Plant Physiol. 2008 Oct;148(2):761-74. Epub 2008 Aug 27.

This was further enhanced by the cytosolic protein synthesis inhibitor cycloheximide but blocked by protease inhibitors, thereby suggesting that the phytohormone acts on the stability of the kinase.
2(0,0,0,2) Details
16983537 Monreal JA, Lopez-Baena FJ, Vidal J, Echevarria C, Garcia-Maurino S: Effect of LiCl on phosphoenolpyruvate carboxylase kinase and the phosphorylation of phosphoenolpyruvate carboxylase in leaf disks and leaves of Sorghum vulgare. Planta. 2007 Mar;225(4):801-12.

The cytosolic protein synthesis inhibitor cycloheximide (CHX), which blocked the light-dependent up-regulation of the kinase in control plants, was found not to be active on this process in preilluminated, LiCl-treated leaves.
2(0,0,0,2) Details
16925599 Shenton M, Fontaine V, Hartwell J, Marsh JT, Jenkins GI, Nimmo HG: Distinct patterns of control and expression amongst members of the PEP carboxylase kinase gene family in C4 plants. Plant J. 2006 Oct;48(1):45-53. Epub 2006 Aug 22.

We have examined the complexity of the phosphoenolpyruvate carboxylase kinase (PPCK) gene family in the C (4) monocots maize and sorghum.
ZmPPCK2, ZmPPCK3 and ZmPPCK4 are all induced by exposure of tissue to cycloheximide, whereas ZmPPCK1 is not.
2(0,0,0,2) Details