| Name | cyclin dependent kinase (protein family or complex) |
|---|---|
| Synonyms | cyclin dependent kinase; cyclin dependent kinases |
| Name | cycloheximide |
|---|---|
| CAS |
| PubMed | Abstract | RScore(About this table) | |
|---|---|---|---|
| 15870436 | Van Dross R, Yao S, Asad S, Westlake G, Mays DJ, Barquero L, Duell S, Pietenpol JA, Browning PJ: Constitutively active K-cyclin/cdk6 kinase in Kaposi sarcoma-associated herpesvirus-infected cells. J Natl Cancer Inst. 2005 May 4;97(9):656-66. BACKGROUND: Kaposi sarcoma-associated human herpesvirus (KSHV) encodes K-cyclin, a homologue of D-type cellular cyclins, which binds cyclin-dependent kinases to phosphorylate various substrates. The half-life of K-cyclin and cyclin D2 proteins was determined by blocking protein synthesis with cycloheximide and measuring proteins in cell lysates by western blot analysis. |
2(0,0,0,2) | Details |
| 17407140 | Fu Y, Fang Z, Liang Y, Zhu X, Prins P, Li Z, Wang L, Sun L, Jin J, Yang Y, Zha X: Overexpression of integrin beta1 inhibits proliferation of hepatocellular carcinoma cell SMMC-7721 through preventing Skp2-dependent degradation of p27 via PI3K pathway. J Cell Biochem. 2007 Oct 15;102(3):704-18. Here we reported that one of the cyclin-dependent kinase (CDK) inhibitors, p27 (Kip1) was involved in proliferation-inhibition induced by overexpression of integrin beta1. Cycloheximide (Chx) treatment and pulse-chase experiments revealed that overexpression of integrin beta1 prolonged the half-life of p27 (Kip1) by inhibiting its degradation. |
1(0,0,0,1) | Details |
| 16452462 | Jelinkova L, Kubelka M: Neither Aurora B activity nor histone H3 phosphorylation is essential for chromosome condensation during meiotic maturation of porcine oocytes. Biol Reprod. 2006 May;74(5):905-12. Epub 2006 Feb 1. Treatment of oocytes with 50 microM I (BL-I), an inhibitor of cyclin-dependent kinases, or cycloheximide (10 microg/ml), inhibitor of proteosynthesis, results in a block of oocytes in the germinal vesicle stage, when nuclear membrane remains intact; however, condensed chromosome fibers or highly condensed chromosome bivalents can be seen in the nucleoplasm of BL-I- or cycloheximide-treated oocytes, respectively. |
31(0,1,1,1) | Details |
| 15545300 | Jaruga-Killeen E, Rayford W: TNF receptor 1 is involved in the induction of apoptosis by the cyclin dependent kinase inhibitor p27Kip1 in the prostate cancer cell line PC-3. FASEB J. 2005 Jan;19(1):139-41. Epub 2004 Nov 15. Cell cycle and typical markers of apoptosis were analyzed by flow cytometry in the presence of the following reagents: cycloheximide, pan-caspase inhibitor ZVAD-fmk, neutralizing anti-TNFR1, and anti-TNFR2. |
2(0,0,0,2) | Details |
| 15753078 | Arbel-Goren R, Levy Y, Ronen D, Zick Y: Cyclin-dependent kinase inhibitors and JNK act as molecular switches, regulating the choice between growth arrest and apoptosis induced by galectin-8. J Biol Chem. 2005 May 13;280(19):19105-14. Epub 2005 Mar 7. When p21 expression was inhibited by cycloheximide, galectin-8 directed the cells toward apoptosis, which involves induction of poly (ADP- polymerase cleavage. |
2(0,0,0,2) | Details |
| 19016568 | Malone JL, Nelson AC, Lieberman R, Anderson S, Holt JT: Oestrogen-mediated phosphorylation and stabilization of BRCA2 protein in breast. J Pathol. 2009 Feb;217(3):380-8. This increase seen in BRCA2 total and phospho-S3291 protein levels was found to be unaffected with cycloheximide pre-treatment, but decreased following tamoxifen, ICI 182,780 or roscovitine treatment. This suggests a requirement for ER and cdk (cyclin-dependent kinase) in mediating the increased protein levels. |
1(0,0,0,1) | Details |
| 17266789 | Toranzo GS, Bonilla F, Zelarayan L, Oterino J, Buhler MI: Activation of maturation promoting factor in Bufo arenarum oocytes: injection of mature cytoplasm and germinal vesicle contents. Zygote. 2006 Nov;14(4):305-16. Reinitiation of meiosis is regulated by maturation promoting factor (MPF), a complex of the cyclin-dependent kinase p34cdc2 and cyclin B. |
1(0,0,0,1) | Details |
| 15964852 | Zhu H, Nie L, Maki CG: Cdk2-dependent Inhibition of p21 stability via a C-terminal cyclin-binding motif. J Biol Chem. 2005 Aug 12;280(32):29282-8. Epub 2005 Jun 17. p21 is a member of the Cip/Kip family of cyclin-dependent kinase (CDK) inhibitors that includes p21, p27, and p57. |
1(0,0,0,1) | Details |
| 18784249 | Ajduk A, Ciemerych MA, Nixon V, Swann K, Maleszewski M: Fertilization differently affects the levels of cyclin B1 and M-phase promoting factor activity in maturing and metaphase II mouse oocytes. Reproduction. 2008 Dec;136(6):741-52. Epub 2008 Sep 10. In metaphase II oocytes, it leads to a Ca (2)(+)-dependent, continuous degradation of cyclin B1 and inactivation of cyclin dependent kinase (CDC2A)-cyclin B1 complex (MPF). |
1(0,0,0,1) | Details |
| 19009649 | Guller M, Toualbi-Abed K, Legrand A, Michel L, Mauviel A, Bernuau D, Daniel F: c-Fos overexpression increases the proliferation of human hepatocytes by stabilizing nuclear Cyclin D1. World J Gastroenterol. 2008 Nov 7;14(41):6339-46. The levels of cell cycle regulatory proteins (Cyclin D1, E, A) cyclin dependent kinases (cdk) cdk2, cdk4, cdk6, and their inhibitors p15, p16, p21, p27, total and phosphorylated GSK-3beta and epidermal growth factor receptor (EGF-R) were assayed by Western blotting. Stability of Cyclin D1 was studied by cycloheximide blockade experiments. |
1(0,0,0,1) | Details |
| 16407831 | Taube ME, Liu XW, Fridman R, Kim HR: TIMP-1 regulation of cell cycle in human breast epithelial cells via stabilization of p27 (KIP1) protein. Oncogene. 2006 May 18;25(21):3041-8. TIMP-1-mediated cell cycle arrest is associated with its downregulation of cyclin D (1) and upregulation of p27 (KIP1), resulting in inhibition of cyclin-dependent kinase activity necessary for phosphorylation of the tumor suppressor retinoblastoma protein. |
1(0,0,0,1) | Details |
| 19424645 | Lee EY, Jeon MJ, Yang W, Shin I: Effect of p27 on motility of MDA-MB-231 breast cancer cells. Oncol Rep. 2009 Jun;21(6):1621-5. p27 is a member of the Kip family of cyclin-dependent kinase inhibitors and its overexpression results in cell cycle arrest at G1 and/or apoptosis. Wound healing assays revealed that migration of MDA-MB-231 hpshRNA cells was markedly impaired compared to MDA-MB-231 mpshRNA cells, especially when cycloheximide was added to block protein synthesis. |
1(0,0,0,1) | Details |
| 17329956 | Matsumoto E, Hatanaka M, Bohgaki M, Maeda S: PKC pathway and ERK/MAPK pathway are required for induction of cyclin D1 and p21Waf1 during 12-o-tetradecanoylphorbol 13--induced differentiation of myeloleukemia cells. Kobe J Med Sci. 2006;52(6):181-94. The upregulation of p21Waf1 seems to play a critical role in TPA-induced cell differentiation by suppressing cyclin dependent kinase activity , while the upregulation of cyclin D1 seems to be compensated by p21Waf1. |
1(0,0,0,1) | Details |
| 18204075 | Cucciolla V, Borriello A, Criscuolo M, Sinisi AA, Bencivenga D, Tramontano A, Scudieri AC, Oliva A, Zappia V, Della Ragione F: Histone deacetylase inhibitors upregulate p57Kip2 level by enhancing its expression through Sp1 transcription factor. Carcinogenesis. 2008 Mar;29(3):560-7. Epub 2008 Jan 19. Here, we evaluate the effects of (BuA) and other HDACIs on p57 (Kip2), a cyclin-dependent kinase inhibitor (cki). The cki upregulation is associated with an increased gene expression that was not prevented by cycloheximide, indicating that HDACIs affect directly p57 (Kip2) transcription. |
1(0,0,0,1) | Details |
| 18721791 | Nam M, Lee WH, Bae EJ, Kim SG: Compound C inhibits clonal expansion of preadipocytes by increasing p21 level irrespectively of AMPK inhibition. Arch Biochem Biophys. 2008 Nov 1;479(1):74-81. Epub 2008 Aug 9. CC treatment blocked hormone-induced preadipocyte differentiation due to inhibition of mitotic clonal expansion, which was accompanied by the failure of degradation of p21, a cyclin-dependent kinase inhibitor. Cycloheximide decreased the basal p21 level, which was inhibited by CC treatment, supporting the stabilization of p21 by CC. |
1(0,0,0,1) | Details |
| 17173074 | Ma Y, Cress WD: Transcriptional upregulation of p57 (Kip2) by the cyclin-dependent kinase inhibitor BMS-387032 is E2F dependent and serves as a negative feedback loop limiting cytotoxicity. Oncogene. 2007 May 24;26(24):3532-40. Epub 2006 Dec 18. In spite of the fact that cyclin-dependent kinase (cdk) inhibiting drugs are potent transcriptional repressors, we discover that p57 (Kip2, CDKN1C) transcription is significantly upregulated by three small molecule cdk inhibitors, including BMS-387032. An E2F1-estrogen receptor fusion protein activated the endogenous p57 promoter in response to hydroxytamoxifen treatment in the presence of cycloheximide. |
1(0,0,0,1) | Details |